PHYLOGENETIC RELATIONSHIPS AMONG GIANT PANDA AND RELATED SPECIES BASED ON RESTRICTION SITE VARIATIONS IN rDNA SPACERS  

大熊猫及其近缘种rDNA序列变异和系统进化关系(英文)

作　　者：兰宏[1,2] 王文[1,2] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室 [2]威斯康星大学人类肿瘤学系

出　　处：《Zoological Research》1998年第5期337-343,共7页动物学研究（英文）

摘　　要：In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.应用ｒＤＮＡ间隔区Ｓｏｕｔｈｅｒｎ转移技术研究大熊猫及其近缘种的分子系统关系。通过比较大熊猫、小熊猫、黑熊、马来熊、浣熊和猞猁的ｒＤＮＡ间隔区限制性内切酶图谱，用最大似然法和简约法构建它们的分子系统树。结果表明大熊猫与熊具有较近的亲缘关系，与小熊猫和浣熊的亲缘关系较远。

关 键 词：Arctoidea The giant panda Ribosomal DNA restriction maps Phylogenetic relationships 

分 类 号：Q959.838[生物学—动物学] Q953