实时荧光PCR检测乙肝病毒DNA的临床意义  

Clinical Significance of the detection of HBV-DNA by the Real-time Fluorescence quantitative PCR

作　　者：俞安清[1] 陈效琴[1] 苏丽萍[1] 何先伟[1] 张韩[1] 

机构地区：[1]广元市中心医院,628000

出　　处：《中国临床实用医学》2010年第1期88-89,共2页China Clinical Practical Medicine

摘　　要：目的用实时荧光定量PCR检测乙型肝炎患者HBV—DNA结果进行分析，以探讨其临床诊疗意义。方法对850例临床标本用时间分辨荧光免疫技术定量测定乙肝病毒血清学指标，用荧光定量PCR法检测血标本中的HBV—DNA，并依据乙肝两对半结果进行归类分组。结果302份HBsAg（＋）、HBeAg（＋）、HBcAb（＋）标本中有261份HBV—DNA为阳性，阳性率为86．4％，其PCR定量拷贝数为（2．21±0．76）×10^7／ml；321份HBsAg（＋）、HBeAb（＋）、HBcAb（＋）标本有96份HBV—DNA阳性，阳性率达到29．91％，PCR定量拷贝数为（1．69±0．98）×10^6／ml；32份HBsAg（＋）、HBcAb（＋）标本中有9份HBV—DNA为阳性，阳性率为28．13％，28份HBeAb（＋）、HBcAb（＋）标本有4份PCRHBV—DNA阳性，阳性率14．29％；11份HBcAb（＋）标本有6份PCRHBV—DNA阳性，阳性率为42．9％；74份HBsAb（＋）、HBeAb（＋）、HBcAb（＋）标本有5份HBV—DNA阳性，阳性率6．75％；13份HBsAb（＋）、HBeAb（＋）阳性标本有2份PCRHBV—DNA阳性，阳性率为14．3％；12份HBsAb（＋）、HBcAb（＋）标本有1份HBV—DNA阳性，阳性率7．69％；4份HBsAb（＋）的标本HBV—DNA均为阴性，PCR定量拷贝数为〈1．00E×103；24份HBsAg（＋）、HBsAb（＋）、HBeAg（＋）、HBcAb（＋）标本中有21份HBV—DNA为阳性，阳性率为87．50％；1份HBsAg（＋）标本HBV—DNA均为阳性，阳性率为100％；10份HBsAg（＋）、HBsAb（＋）、HBeAb（＋）、HBcAb（＋）标本有3份HBV—DNA均为阳性，阳性率30．00％，其余38例全阴性结果和各少见模式基本见有HBV—DNA的检出。结论PCR定量测定HBV—DNA可以真实反映体内乙肝病毒感染和复制及病毒载量情况，更有利于临床治疗和疗效观察。Objective To analyze HBV-DNA results of hepatitis B patients using real-time fluorescence quantitative PCR detection and to explore the significance of their clinical treatment. Methods 850 cases of clinical specimens were detected by time-resolved fluorescence immunoassay quantitative determination and fluorescent quantitative PCR assay detected HBV-DNA of blood samples and the resuhs were divided groups. Results There were 261 positive in group that the HBsAg（ ＋ ） , HBeAg（ ＋ ）and HBcAb （ ＋ ）were positive and positive rate was 86.4% （261/302）and its quantitative PCR for copy number was （2. 21 ± 0. 76） × 10^7/ml. There were 96 positive in group that the HBsAg（ ＋ ）, HBeAb（ ＋ ）and HBeAb（ ＋ ）were positive and the positive rate was 29.91% （96/321）and its quantitative PCR for copy number was（ 1.69 ± 0. 98 ） × 10^6/ml. There were 9 were positive in group that the HBsAg（ ＋ ）and HBeAb（ ＋ ）were positive and positive rate was 28. 13% （9/32）. There were 4 positive in group that the HBeAb （ ＋ ） and HBeAb （ ＋ ） were positive and positive rate was 14.29% （4/28）. There were 6 positive in group that the HBeAb（ ＋ ）was positive and the positive rate was 42. 9% （6/11 ）. There were 5 positive in group that the HBsAb（ ＋ ）, HBeAb（ ＋ ） and HBcAb（ ＋ ） were positive and the positive rate was 6. 75% （5/74）. There were 2 positive in group that HBsAb（ ＋ ）and HBeAb（ ＋ ） positive and the positive rate was 14. 3% （2/13）. There were 1 positive in group that HBsAb（ ＋ ）and HBcAb （ ＋ ） were positive and a positive rate was 7.69% （1/12）. 4 were negative in group of HBsAb（ ＋ ）. There were 21 positive in the group that were HBsAg（ ＋ ）,HBsAb（ ＋ ） ,HBeAg（ ＋ ）and HBcAb（ ＋ ）were positive and the positive rate was 87.50% （21/24）. The positive rate was 100% l in group of HBsAg （ ＋ ）. There were 3 positive in group that HBsAg（ ＋ ）, HBsAb（ ＋ ） , HBeAb（ ＋

关 键 词：乙肝血清标志物 时间分辨荧光免疫技术 荧光定量PCR技术 临床应用 

分 类 号：R4[医药卫生—临床医学] R51