偏瘫康复丸对局灶性脑缺血大鼠再灌注(MCAO)后脑组织氧自由基和NOS表达的影响  

The Effects of the Piantan Kangfu Pill on Brain Tissue Free Radicals and Nitric Oxide Synthase(NOS)Expression following Focal Cerebral lschemia/Reperfusion Injury

作　　者：孙菊光 朱萱萱[2] 达庆国[2] 吴旭彤[2] 秦晓康[3] 

机构地区：[1]徐州中医院,徐州221002 [2]南京中医药大学附属医院,南京210029 [3]南京中医药大学,南京210029

出　　处：《中国医药导刊》2010年第1期82-84,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:观察偏瘫康复丸对大鼠局灶性脑缺血-再灌注损伤脑组织自由基及一氧化氮合成酶(NOS)变化的影响。方法:用线栓法制备大鼠局灶性脑缺血再灌注损伤模型,检测丙二醛(MDA)、超氧化物歧化酶(SOD)及一氧化氮合成酶(NOS)。结果缺血再灌注损伤后,大鼠海马神经元细胞凋亡随时间延长而增加,脂质过氧化产物丙二醛含量和一氧化氮合酶活性增高。结果:偏瘫康复丸干预可显著减轻海马神经元凋亡,降低MDA含量和NOS活性,SOD含量升高。结论:在脑缺血再灌注损伤后,偏瘫康复丸能抑制脑缺血再灌注后脂质过氧化反应,对抗自由基损伤,促进自由基清除,提高脑组织自身抗氧化能力,保护脑细胞。Objective： To observe the effects of the Piantan Kangfu pili on brain tissue free radicals and nitric oxide synthase （NOS） changes following focal cerebral ischemia/reperfusion injury.Methods： Used intraluminal filament occlusion to prepared the focal cerebral ischemia/reperfusion injury model, detected malondialdehyde （MDA）, superoxide dismutase tSOD） and nitric oxide synthase （NOS）. After ischemia-reperfusion injury rat hippocampal neuronal apoptos^s increased with time. lipid peroxidation malondialdehyde and nitric oxide synthase activity increased.Results： Piantan Kangfu pill can significantly reduce the hippocampal neuronal apoptosis, degrade MDA content and NOS activity, increased SOD content.Conclusion： Following cerebral ischemia/reperfusion injury, Piantan Kangfu pill can inhibit brain lipid peroxidation, fight against free radical damage, promote free radical scavenging and improve brain tissue antioxidant capacity, protect brain cells.

关 键 词：脑缺血-再灌注损伤大鼠 SOD MDA NOS 

分 类 号：R285.5[医药卫生—中药学]