六种蜂花粉的红外光谱三级鉴别研究  

Study on the Identification of Six Kinds of Bee Pollens by Three-Step Infrared Macro-Fingerprint Method

作　　者：吴杰[1,2] 周群[3] 吴黎明[1] 安建东[1] 孙素琴[3] 胡福良[2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]浙江大学动物科学学院,浙江杭州310029 [3]清华大学化学系生命有机磷化学及化学生物学教育部重点实验室,北京100084

出　　处：《光谱学与光谱分析》2010年第2期353-357,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30771636);现代农业(蜂)产业技术体系岗位科学家科研经费项目资助

摘　　要：采用傅里叶变换红外光谱(FTIR)结合二阶导数谱和热扰动下的二维相关红外光谱技术对6种不同花粉,即杏花花粉、油菜花粉、茶花花粉、西瓜花粉、荷花花粉和虞美人花粉,进行了快速无损的鉴别。结果表明,在一维红外光谱图上,不同花粉的蛋白质、脂肪和糖类物质的特征吸收峰在相对峰强和峰位上均存在一定的差异,在二阶导数谱上差异很明显。而在二维红外谱图上,由于6种花粉的自动峰及相关峰峰簇的位置和数量不同,其差别体现得更为明显和直观。因此,三级红外宏观指纹图谱法是鉴别不同蜂花粉种类的一种有效和快速检测方法。Six kinds of bee pollens, including apricot pollen, lotus pollen, rape pollen, camellia pollen, watermelon pollen and corn poppy pollen, were identified non-destructively by Fourier transform infrared spectroscopy （FTIR） combined with deriva- tive spectra and two dimensional correlation spectroscopy （2D） in the present article. Compared with conventional IR spectra of samples, some certain differences were found in the characteristic peaks of proteins, lipids and carbohydrates. Obvious differ- ences of 1he six kinds of bee pollens were found in the second derivative spectra. And in the 2D-IR correlation spectra, the sam- ples presented the differences in the position and intensity of the autopeaks and correlation peak clusters. Therefore, the three- step IR macro〉fingerprint provides a more rapid and effective method for the identification of different kinds of bee pollens.

关 键 词：蜂花粉 红外光谱 二维相关红外光谱 鉴别 

分 类 号：O657.3[理学—分析化学]