rhBMP-7对腺嘌呤致大鼠慢性肾衰竭防治作用的初步研究  

Preventive and therapeutic effects of rhBMP-7 on chronic renal failure induced by adenine in rats

作　　者：迟名锋[1] 韩金祥[1] 王世立[1] 郑秀峰[2] 苑广盈[2] 

机构地区：[1]山东省医药生物技术研究中心,卫生部生物技术药物重点实验室,济南250062 [2]济南市第四人民医院,济南250031

出　　处：《中国新药杂志》2010年第2期157-160,146,共5页Chinese Journal of New Drugs

基　　金：国家高技术研究发展计划(863计划)(2003AA2Z3532)

摘　　要：目的:探讨rhBMP-7对腺嘌呤致大鼠慢性肾衰竭的防治作用。方法:将Wistar大鼠随机分为正常组、模型组、模型对照组、rhBMP-7预防组、rhBMP-7小、大剂量治疗组。除正常组外,其余各组按300mg.kg-1.d-1用腺嘌呤灌胃,rhBMP-7预防组在灌胃的同时按300μg.kg-1隔日腹腔注射rhBMP-7。在d 35各组停止灌胃并处死正常组、模型组、rhBMP-7预防组的大鼠,取血,作生化指标及血液指标检测,并留取肾组织。从d 36开始,模型对照组按1 mL.kg-1的剂量隔日腹腔注射生理氯化钠溶液,rhBMP-7小、大剂量治疗组分别以100和300μg.kg-1隔日腹腔注射rhBMP-7。在d 70处死大鼠,取血,作生化指标及血液指标检测,并留取肾组织。结果:rhBMP-7在不同程度上降低慢性肾衰大鼠的BUN,Cr,UA,磷含量,增加钙含量,改善贫血,降低肾脏指数,减轻肾衰大鼠的肾脏损伤。结论:rhBMP-7对腺嘌呤致大鼠慢性肾衰竭有一定的防治作用,为探索治疗肾功能衰竭提供新的途径。Objective： To observe the preventive and therapeutic effects of recombinant human bone morphogenetic protein-7 （rhBMP-7） on chronic renal failure induced by adenine in rats. Methods： Chronic renal failure was induced by intragastric adenine （300 mg· kg^-1·d^-1 ） for 35 days in Wistar rats. For the preventive effects, rhBMP-7 （300 μg·kg^-1 ） was ip injected every other day during adenine treatment. At 35 days after treatment, renal function, biochemical indicators, kidney index and renal pathologic changes were determined. For the therapeutic effects, rhBMP-7 （ 100 and 300 μg·kg^-1） was ip injected every other day from 36 days of adenine treatment to 70 days. On 70th day, the same indicators were observed. Results： rhBMP-7 declined the levels of BUN, Cr, UA and P^3＋ , and increased the level of Ca^2＋ somewhat; it also improved anemia, reduced the renal index and attenuated the injury of kidney. Conclusion： rhBMP-7 improves the renal dysfunction in rats with chronic renal failure induced by adenine.

关 键 词：重组人骨形态发生蛋白-7 慢性肾衰竭 腺嘌呤 

分 类 号：R965[医药卫生—药理学]