1517例乙肝标志物和HBV-DNA定量相关性分析  

Correlation Analysis Between HBV Markers and HBV-DNA Based on 1517 Cases

作　　者：邢颖[1] 程金华[1] 高勇[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院检验科,黑龙江齐齐哈尔161000

出　　处：《中华医院感染学杂志》2010年第4期502-503,共2页Chinese Journal of Nosocomiology

摘　　要：目的探讨血清HBV-DNA水平与HBV血清标志物的相关性。方法采用实时荧光定量PCR对1517例HBV感染者血清标本中HBV-DNA含量进行检测,同时用ELISA法检测其HBV免疫标志物。结果HBsAg、HBeAg、HBcAb阳性组患者血清HBV-DNA检出率98.24%;HBsAg、HBeAg阳性组为100.00%;HBsAg、HBeAb、HBcAb阳性组为60.98%;HBsAg、HBcAb阳性组为37.71%;HBcAb阳性组为12.90%。结论患者血清HBV-DNA的阳性率与HBV血清标志物的存在状态相关,采用FQ PCR法检测HBV-DNA能更准确、直接地反映体内病毒复制情况。OBJECTIVE To investigate the relationship between the level of HBV-DNA and Serum markers. METHODS FQ PCR was used to detect the contents of serum HBV-DNA in 1517 patients infected HBV and ELISA was used to detect the immunological markers. RESULTS The detected rate of HBsAg（＋）, HBeAg（＋）, HBcAb（＋） group were 98.24%, HBsAg（＋）, HBeAg（＋） group 100.00% of HBsAg（＋）, HBeAb（＋）, HBcAb（＋） group 60.98 %, of HBsAg（＋）, HBcAb（＋） showed a HBV-DNA group 37.71%, and of HBcAb（＋） showed a HBV-DNA group was 12.90%. CONCLUSIONS The positive rate of serum HBV-DNA is related to the mode of serum HBV markers. The result of serum HBV-DNA detected by FQ-PCR can reflect the replication of hepatitis B virus more correctly and directly.

关 键 词：乙型肝炎病毒 实时荧光PCR 血清标志物 

分 类 号：R512.62[医药卫生—内科学]