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作 者:曹烁晖[1] 谢堂堂[1] 蔡伟鹏[1] 李耀群[1]
机构地区:[1]厦门大学化学化工学院化学系与现代分析科学重点实验室,厦门361005
出 处:《高等学校化学学报》2010年第1期61-63,共3页Chemical Journal of Chinese Universities
基 金:国家自然科学基金(批准号:20975084;20575055);国家"九七三"计划项目(批准号:2007CB935600)资助
摘 要:The observation of surface plasmon-coupled directional fluorescence(SPCDF) on thin iron films was presented. SPCDF from thin iron films was p-polarized with a directional emission angle of 70°. Fluorescein and meso-tetra(4-sulfonatophenyl)porphine(TPPS) were used as a model system of dual fluorophores. Using 25 nm thin iron films,the SPCDF signals of the two fluorophores were observed at a fixed angle just by one scan. The SPCDF signals of Fluorescein and TPPS added to whole blood were identified clearly,eliminating the background interference of blood effectively. Thin iron films have been proven to be new materials for SPCDF detection,promoting a new mode to observe the enhanced fluorescence signals of different fluorophores by one scan. They should be useful for tracking the labled systems of multi-fluorophores in biological applications.The observation of surface plasmon-coupled directional fluorescence (SPCDF) on thin' iron films was presented. SPCDF from thin iron films was p-polarized with a directional emission angle of 70°. Fluorescein and meso-tetra(4-sulfonatophenyl)porphine (TPPS) were used as a model system of dual fluorophores. Using 25 nm thin iron films, the SPCDF signals of the two fluorophores were observed at a fixed angle just by one scan. The SPCDF signals of Fluorescein and TPPS added to whole blood were identified clearly, eliminating the background interference of blood effectively. Thin iron films have been proven to be new materials for SPCDF detection, promoting a new mode to observe the enhanced fluorescence signals of different fluorophores by one scan. They should be useful for tracking the labled systems of muhi-fluorophores in biological applications.
关 键 词:表面等离子体耦合定向荧光 铁薄膜 定向角 双荧光团
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