天然棕色棉查尔酮合成酶基因(GhCHS1)的克隆和实时定量表达分析  

Cloning and Quantitative Analysis by Real-Time RT-PCR of a Chalcones Synthase Gene(GhCHS1)in the Natural Brown Cotton Fibers

作　　者：杨会娜[1] 田新惠 李艳军[1] 李明月[1] 冯鸿杰[1] 孙杰[1] 

机构地区：[1]石河子大学农学院/新疆兵团绿洲生态农业重点实验室,石河子832003

出　　处：《棉花学报》2010年第1期42-48,共7页Cotton Science

基　　金：国家973前期专项(2007CB116210);兵团科技局基础研究项目(2006JC02)

摘　　要：根据植物查尔酮合成酶基因保守区序列设计引物,以发育18d的天然棕色棉纤维为材料,用RT-PCR结合RACE技术分离得到了一个查尔酮合成酶基因的全长cDNA(GenBank登录号:EU921263),将该基因命名为GhCHS1。实时荧光定量PCR检测显示,GhCHS1基因在棕色棉纤维细胞中优先表达,且在棕色棉纤维中的表达量远高于其近等基因系白色棉,但是该基因在绿色棉中几乎检测不到。这些试验结果暗示,该基因可能在棕色棉纤维色素形成中发挥重要作用。A full-length cDNA encoding CHS was cloned from brown cotton fiber of 18 days post anthesis by RT-PCR and rapid amplification ofcDNA ends （RACE） using specific primers based on the highly conserved sequences of plant CHS that had already known. Blast search revealed that it is a new gene, and was named as GhCHS1 （GenBank accession： EU921263）. The temporal and spatial expression patterns of GhCHS1 gene were examined by the real-time quantitative PCR with the specific primers. The expression analysis indicated that GhCHS1 gene was preferentially expressed in brown cotton fibers during development, and the expressed levels of GhCHS1 gene in white fibers and green fibers were very low or undetectable, which suggested that GhCHS1 gene might play an important role in brown pigmentation of naturally brown cotton fibers.

关 键 词：查尔酮合成酶 天然彩色棉 实时定量RT—PCR 

分 类 号：S562.035[农业科学—作物学]