适宜水稻花粉萌发的液体培养基的优化  

Screening for Ingredients of a Liquid Culture Medium for Rice Pollen Germination Test

作　　者：温婵[1] 罗海伟[1] 彭富海[1] 王生银[1] 田小海[1] 

机构地区：[1]长江大学农学院,湖北荆州434025

出　　处：《中国农学通报》2010年第2期68-70,共3页Chinese Agricultural Science Bulletin

基　　金：长江大学国家大学生创新性实验计划项目(081048914);国家自然科学基金资助项目(30671218)

摘　　要：花粉萌发率是检测花粉活力的一个重要指标,但实践中还缺少便捷、可靠的水稻花粉萌发率测定方法。作者在前人研究的基础上,以两系杂交中稻两优818为试验材料,采用液体培养基对相关培养条件进行了优化。结果表明,采用155 g/L蔗糖+20 mg/L H3BO3+40 mg/L CaCl2+5 mg/L V-B1相配合的培养基较适合花粉的萌发,萌发率最高可达63.78%。此外,该培养方法具有培养条件要求不高、发芽结果稳定性好、培养基分布均匀等优点。The pollen germination rate （PGR） is one of the most important factors to evaluate the pollen vltahty. Practically, low-cost, high-speed and feasible culture methods for determination of PGR are lacking. With a two-line hybrid rice cultivar LiangyouS18, we＂ ve tried to screen the ingredieats of a liquid culture medium for rice pollen germination test based on some previous studies. The results indicated that the culture medium with a prescription of 155 g/L sucrose ＋20 mg/L H3BO3＋40 mg/L CACl2＋5 mg/L VB1 showed significantly greater than other ingredient-based media, with a highest PGR 63.78%. In addition, this culture method was characterized by low-cost, stability in PGR, and even distribution of pollen grains on the surface of the culture medium.

关 键 词：水稻 花粉 液体培养基 萌发率 

分 类 号：S131.2[农业科学—农业基础科学]