双异丙酚预处理对内毒素诱导的人中性粒细胞释放白细胞介素-8和肿瘤坏死因子-α及相应mRNA表达的影响  

Effects of pretreatment with propofol on the release of TNF - α, IL - 8 and TNF - α mRNA, IL- 8 mRNA expression of polymorphonuclear leukocytes stimulated by lipopolysaccharide

作　　者：池信锦[1] 程楠[1] 黑子清[1] 罗刚健[1] 罗晨芳[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广州510630

出　　处：《中国急救医学》2010年第1期65-68,共4页Chinese Journal of Critical Care Medicine

基　　金：广东省自然科学基金项目（No.7001567）;广东省科技计划项目（No.20078031511009）

摘　　要：目的探讨双异丙酚预处理对内毒素（LPS）诱导的健康成年人静脉血中性粒细胞（polymorphonuclear leukocyte，PMN）释放白细胞介素（interleukin，IL）-8和肿瘤坏死因子（tumor necrosis factor，TNF）-α的影响及其应mRNA表达的变化。方法采集健康成人志愿者静脉血分离提纯PMN，用1640培养液制成悬液；试验分为6组：Ⅰ组（空白对照组，PMN＋培养液），Ⅱ组（双异丙酚溶剂对照组，intralipid），Ⅲ组（双异丙酚组，终浓度5mg／L双异丙酚），Ⅳ组（内毒素组，终浓度1mg／L LPS），V组（内毒素＋溶剂对照组，intralipid＋终浓度1mg／L LPS），Ⅳ组（双异丙酚＋内毒素组，终浓度5mg／L双异丙酚＋终浓度1mg／L LPS）。加药培养12h后，ELISA法检测培养上清液TNF-α、IL-8的浓度，荧光定量PCR检测PMN IL-8 mRNA、TNF-α mRNA表达量。结果与Ⅰ组比较，Ⅳ组、Ⅴ组、Ⅵ组IL-8浓度升高，Ⅳ组TNF-α浓度升高（P〈0．05）；与Ⅳ组比较，Ⅵ组IL-8和TNF-α浓度降低（P〈0．05）。与Ⅰ组比较，Ⅳ组、Ⅴ组、Ⅵ组IL-8 mRNA表达上调，Ⅳ组TNF-α mRNA表达上调（P〈0．05）；与Ⅳ组比较，Ⅵ组IL-8 mRNA和TNF-α mRNA表达下调（P〈0．05）。结论双异丙酚预处理可通过抑制内毒素诱导的入PMN释放IL-8、TNF-α，下调IL-8、TNF-α的mRNA表达，对PMN介导的炎症反应具有抑制作用。Objective To observe the effects of pretreatment with propofol on releasing tumor necrosis factor（TNF） - α, interleukin （IL） - 8 and TNF - α mRNA, IL - 8 mRNA expression of polymorphonuclear leukocytes （PMNs） stimulated by lipopolysaeeharide in vitro. Methods PMNs were separated from the whole blood obtained from healthy adult and incubated in RPMI1640 cell culture medium. The cultured PMNs were divided into six groups randomly： group Ⅰ （control group, cell culture medium was added ） , group Ⅱ （ intralipid group, 10% intralipid was added ） , group HI （propofol group, final concentration of 5mg/L propofol was added ） , group Ⅳ （ LPS group, final concentration of 1mg/L LPS was added）, group Ⅴ （LPS ＋ intralipid group, 10% intralipid and final concentration of 1 mg/L LPS was added ） , groupVI （LPS ＋ propofol group, final concentration of 5mg/L propofol and lmg/L PLS was added）. Concentration of TNF - α and IL - 8 in supernatant fluid was detected by ELISA, and expression of TNF -α mRNA and IL - 8 mRNA of PMNs were detected by fluorescent quantitation polymerase chain reaction （Q -PCR ）. Results Compared with group Ⅰ ,concentration of IL - 8 was increased in group Ⅳ, Ⅴ and Ⅵ; and concentration of TNF - α was increased in group Ⅳ （P 〈 0.05 ）. Compared with group Ⅳ, concentration of IL - 8 and TNF - α was reduced in group Ⅵ（P 〈 0.05）. Compared with group Ⅰ , IL - 8 mRNA expression was up - regulated in group Ⅳ, Ⅴ and Ⅵ ; and TNF - α mRNA expression was up - regulated in group Ⅳ （ P 〈 0.05 ）. Compared with group Ⅳ, expression of IL - 8 mRNA and TNF - α mRNA was down - regulated in group Ⅵ （P 〈 0.05 ）. Conclusion Release of IL - 8 and TNF - α and expression of IL - 8 mRNA and TNF - α mRNA of PMNs stimulated by lipopolysaccharide were inhibited by pretreatment with propofol. Propofol had the inhibitions on inflammatory response mediated by PMNs.

关 键 词：内毒素 中性粒细胞 双异丙酚 白细胞介素-8 肿瘤坏死因子-Α 

分 类 号：R259.752[医药卫生—中西医结合] R587.1[医药卫生—中医内科学]