HPLC法测定蚕桑资源中1-脱氧野尻霉素的含量  

Determination of 1-Deoxynojirimycin in Mulberry Resources by HPLC

作　　者：陈岩[1] 王智勇[2] 杨一坤[1] 

机构地区：[1]哈尔滨医科大学第一附属医院药学部,哈尔滨市150001 [2]哈尔滨医科大学第二附属医院药学部,哈尔滨市150001

出　　处：《中国药房》2010年第3期245-247,共3页China Pharmacy

摘　　要：目的:建立蚕桑资源桑白皮、蚕砂、桑叶中1-脱氧野尻霉素的定量分析方法。方法:采用高效液相色谱-紫外检测器,色谱柱为Hypersil C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%冰醋酸水溶液(50∶50),流速为1.0mL·min-1,检测波长为265nm,柱温为35℃。结果:1-脱氧野尻霉素的检测浓度在5.0×10-4～1.0×10-3mg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为97.7%(RSD=2.0%,n=6)。结论:本法简便可行、结果准确、重复性好,可用于蚕桑资源的质量控制。OBJECTIVE：To establish a quantitative analysis method to determine the contents of 1-deoxynojirimycin in mulberry resources such as Morus alba,Excrementum bombycis,Folium mori.METHODS：l-deoxynojirimycin in mulberry resources were determined by RP-HPLC method and UV detector on Hypersil C18 （250mm×4.6mm,5μm）column, using acetonitrile-0.1% glacial acetic acid（50 ： 50）as moble phase. The flow rate was 1.0 mL·min^-1 and the detection wavelength were set at 265 nm with column temperature at 35℃. RESULTS：The linear range of 1-deoxynojirimycin were from 5.0×10^-4 mg·mL^-1 to 1.0×10^-3 mg·mL^-1 （r= 0.999 9）with recoveries of 97.7% （RSD=2.0%, n=6）.CONCLUSION： The method is simple, accurate and reproducible for the quality control of Mulberry resources.

关 键 词：1-脱氧野尻霉素 桑白皮 蚕砂 桑叶 高效液相色谱法 紫外检测器 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]