HPLC法测定肠安康颗粒中白术内酯Ⅰ与苍术酮的含量  

Determination of AtractylenolideⅠ and Atractylone in Chang’ankang Granule by HPLC

作　　者：何雄伟[1] 

机构地区：[1]重庆市第九人民医院,重庆市400700

出　　处：《中国药房》2010年第3期249-250,共2页China Pharmacy

摘　　要：目的:建立肠安康颗粒中白术内酯Ⅰ与苍术酮的含量测定方法。方法:采用高效液相色谱法,色谱柱为伊利特ODS色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1mL·min-1,检测波长为220nm。结果:白术内酯Ⅰ进样量在0.1～2.0μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均回收率为98.84%,RSD=0.76%(n=6);苍术酮进样量在0.5～10.0μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均回收率为98.43%,RSD=1.14%(n=6)。结论:本方法简便、准确,可用于肠安康颗粒的质量控制。OBJECTIVE： To develop a method for determination of atractylenolide I and atractylone in Chang＇ ankang granule by HPLC. METHODS： The determination was performed on Yilite ODS column （250 mm×4.6 mm, 5 μm）. The mobile phase conmined of acetonitrile-water （gradient elution） with flow rate of 1 mL·min^-1 and detection wavelength of 220 nm. RESULTS： The linear range of atractylenolide I were 0.1-2.0 μg（r=0.999 9） with mean recovery of 98.84% （RSD=0.76% ,n=6）.The linear range of atractylone were 0.5-10.0 μg with mean recovery of 98.43% （RSD=1.14% ,n=6）. CONCLUSION： This method is simple, convenience and accurate for quality control of Chang＇ ankang granule.

关 键 词：肠安康颗粒 白术内酯I 苍术酮 高效液相色谱法 含量测定 

分 类 号：R283.627[医药卫生—中药学] R927.2[医药卫生—中医学]