Graves病患者外周血CD4^＋CD25^＋T细胞及Foxp3mRNA表达的变化  

The change of CD4^＋CD25^＋T cells and the expression of Foxp3 mRNA in peripheral blood of patients with Graves＇ disease

作　　者：查兵兵[1] 刘军[1] 洪晓武[2] 查英[1] 王芳[1] 丁和远[1] 陈灶萍[1] 

机构地区：[1]复旦大学上海医学院上海市第五人民医院内分泌科,上海200240 [2]复旦大学上海医学院免疫学系

出　　处：《临床内科杂志》2010年第1期37-39,共3页Journal of Clinical Internal Medicine

基　　金：复旦大学青年骨干基金资助项目（07L-1）

摘　　要：目的通过比较Graves病患者和正常人外周血中CD4^＋和CD8^＋T细胞占淋巴细胞、CD4^＋CD25^＋T细胞占CD4^＋T细胞的比例以及相关基因Foxp3mRNA表达的变化，探讨Graves病患者免疫机制中CD4^＋CD25^＋T细胞亚群所起的作用。方法选择40例初次确诊Graves病的患者和40名健康自愿者，通过流式细胞仪检测外周血CD4^＋CD8^＋T细胞占淋巴细胞、CD4^＋CD25^和CD4^＋CD25^high细胞占CD4^＋T细胞的比例以及CD4^＋CD25^＋T细胞的荧光强度。应用Real—Time PCR检测外周血单个核细胞Foxp3mRNA的表达。结果Graves病患者外周血中CD4^＋T细胞比例以及CD4^＋／CD8^＋的比值明显升高，而CD4^＋CD25^和CD4^＋CD25^highT细胞占CD4^＋T细胞的比例以及CD4^＋CD25^＋T细胞的荧光强度与正常人比较差异无统计学意义，Graves病患者外周血单个核细胞Foxp3mRNA的表达也无明显减少。结论Graves病主要通过效应性CD4^＋T细胞的体液免疫反应对甲状腺组织产生影响，CD4^＋CD25^＋T细胞的免疫抑制作用减弱可能仅是Graves病发病机制中的一个次要环节。Objective To investigate the role that CD4^＋CD25^＋T cells play in Graves＇ disease by comparing the proportion of CD4^＋CD25^＋T cells to CD4^＋ T cells as well as CD4^＋ and CD8^＋ T cells to lymphocytes in peripheral blood between newly diagnosed patients with Graves＇ disease and healthy people. Methods Flow cytometry was used to detect the proportion of CD4^＋ and CD8^＋ T cells to lymphocytes,the ratio of CD4^＋/CD8^＋, the proportion of CD4^＋CD25^＋, C4^＋CD25^highT cells to CD4^＋ T ceils as well as the mean flu- orescence intensity（MFI） of CD25 expression of these cells respectively in peripheral blood of 40 newly diagnosed patients with Graves＇ disease. Real-time polymerase chain reaction was performed also to measure the expression of Foxp3 mRNA. Results A significant increase in the proportion of CD4^＋ T cells to lymphocytes and the ratio of CD4^＋/CD8^＋ was detected in peripheral blood of newly diagnosed patients with Graves＇ disease. However no significant statistical difference was found in the proportion of CD4^＋CD25^＋T, CD4^＋ CD25^high T cells to CD4^＋ T cells, MFI of CD25 expression as well as the expression of Foxp3 mRNA between the patients and healthy people. Conclusions Graves＇ disease was mainly mediated by CD4^＋ T ceils. CD4^＋CD25^＋T cells may not take an important role in the pathogenesis of Graves＇ disease.

关 键 词：GRAVES病 CD4^+CD25^+T细胞 FOXP3 

分 类 号：R581.1[医药卫生—内分泌]