HPLC测定愈骨疗伤胶囊中川续断皂苷Ⅵ的含量  

Determination of Asperosaponin Ⅵ in Yugu Liaoshang Capsule by HPLC

作　　者：陈海飞[1] 孙艳[1] 卞俊[1] 宣伟东[1] 

机构地区：[1]解放军第411医院南京军区药学专科中心,上海200434

出　　处：《中国现代应用药学》2010年第1期57-58,共2页Chinese Journal of Modern Applied Pharmacy

基　　金：南京军区医学科技创新重点课题(07Z002)

摘　　要：目的采用高效液相法测定愈骨疗伤胶囊中川续断皂苷Ⅵ的含量。方法色谱柱Kromasil ODS-1C18柱(4.6mm×250mm,5μm),流动相:乙腈-水(30∶70),检测波长:210nm,流速:1mL·min-1。结果川续断皂苷Ⅵ的线性范围是0.4～3.28μg(r=0.9995),回收率103.4%,RSD=1.76%。结论该方法简便快捷,结果准确可靠,可作为愈骨疗伤胶囊的质量控制手段。OBJECTIVE To determine asperosaponin Ⅵ in Yugu Liaoshang capsule by HPLC.METHODS The chromatography procedure was carried out using Kromasil ODS-1 C18（4.6 mm×250 mm,5 μm） as an analytic column and a mixture of 30 volume of acetonitrile,70 volume of water as a mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm.RESULTS Asperosaponin Ⅵ line range was between 0.4 μg and 3.28 μg（r=0.999 5）.The average recovery and RSD of asperosaponin Ⅵ were 103.4% and 1.76%.CONCLUSION The method is simple,accurate and reliable,and can be used for the quality assess of Yugu Liaoshang capsule.

关 键 词：愈骨疗伤胶囊 高效液相色谱法 川续断皂苷Ⅵ 

分 类 号：R917.101[医药卫生—药物分析学]