美洛昔康对铝负荷致大鼠海马神经元损伤的保护作用  

Protective Effect of Meloxicam on Aluminum Overload-inducing Damage of Hippocampal Neuron in Rats

作　　者：谢灵瑶[1] 杨俊卿 黄砚[1] 

机构地区：[1]重庆医科大学药学院药理学教研室,重庆市400016

出　　处：《中国药房》2010年第5期400-403,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(30672211)

摘　　要：目的:观察美洛昔康对铝负荷致海马神经元损伤的保护作用。方法:离体培养新生SD大鼠海马神经元7d,分成空白对照组(200μmol·L-1NaCl)、铝模型组(200μmol·L-1AlCl3)、美洛昔康小、中、大剂量(10-8、10-7、10-6mol·L-1)组,给药24h后,检测各组神经元光密度值、乳酸脱氢酶(LDH)漏出率、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并观察神经元形态。结果:与空白对照组比较,铝模型组光密度值降低、LDH漏出率升高、SOD活性减弱、MDA含量升高(P<0.01),神经元形态发生明显损伤;与铝模型组比较,美洛昔康各剂量组光密度值升高、LDH漏出率降低、SOD活性增强、MDA含量降低(P<0.01或P<0.05),神经元形态出现明显改善。结论:美洛昔康对于铝负荷致离体海马神经元损伤有一定的保护作用,其机制可能与抑制炎症反应和氧化应激有关。OBJECTIVE： To investigate the protective effect of meloxicam on aluminum overload-inducing damage of hippocampal neuron. METHODS： Primary hippocampal neuron attained from new born SD rats was cultured for 7 days. There were 5 groups e.g blank control group （200 μmol·L^-1 NaCl）, aluminum model group （200 μmol·L^-1 AlCl3） and meloxicam low-dose （10^-8 mol·L^-1）, medium-dose （10^-7 mol·L^-1） and high-dose （10^-6 mol·L^-1） groups. HE staining was used to observe the change of neuronal morphology. The optical densities of cells were measured. The activities of SOD, leakage of LDH and content of MDA were also detected, RESULTS： As compared with control group, the optical density of cells and activity of SOD were decreased and leakage of LDH and content of MDA were increased （P〈0.01） in aluminum model group. Compared with aluminum model group, the increase in optical density and activity of SOD and decrease in leakage of LDH and content of MDA were observed in meloxican group （P〈0.01 or P〈0.05）. The neuronal morphology was improved remarkably. CONCLUSIONS： Meloxicam has a significant protective effect against neuronal damage induced by aluminum overload. The mechanism may associate with the inhibition of anti-inflammation reaction and anti-oxidative stress.

关 键 词：海马神经元 大鼠 环氧化酶-2 ALCL3 美洛昔康 

分 类 号：R965[医药卫生—药理学] R971.1[医药卫生—药学]