紫龙金对BGC-823细胞增殖的抑制作用及对p16^INK4a启动子活性的影响  

EFFECTS OF ANTICANCER MEDICINE ZILONGJIN ON CELL PROLIFERATION AND ACTIVITY OF p16^(INK4a) GENE PROMOTER IN BGC-823 CELLS

作　　者：樊粉霞[1] 卫玮[1] 柳惠图[1] 张伟[1] 

机构地区：[1]北京师范大学生命科学学院,细胞增殖及调控生物学教育部重点实验室,北京100875

出　　处：《北京师范大学学报（自然科学版）》2010年第1期68-71,共4页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30300173);国家高技术研究发展计划资助项目(2006AA02Z4A6);北京市优秀人才培养资助项目(20071D0503100293);细胞增殖与调控生物学教育部重点实验室开放资助项目

摘　　要：利用记数法和Brdu脉冲标记法研究了紫龙金对细胞增殖的影响,western blot检测结果表明:紫龙金(3,4mg.mL-1)处理可明显抑制人胃癌BGC-823细胞的生长、提高BGC-823细胞中p16的表达水平.进一步利用含有p16INK4a启动子片段(-967^-165区域)及荧光素酶报告系统的载体pGL3-Basic-p16N研究了紫龙金对p16INK4a启动子活性的影响及其作用的分子机制,结果表明,紫龙金可能通过提高p16INK4a启动子活性而促进p16的表达,从而抑制细胞的增殖.To investigate antitumor-related molecular processes in which Zilongjin （ZLJ） involved, the effect of ZLJ on the proliferation of BGC-823 cells and the level of p16^INK4a are assayed respectively by growth curves, BrdU-incorporation assay and western blotting. These results show that ZLJ inhibits proliferation of BGC-823 cells. The level of p16^INK4a increases greatly. The effect of ZLJ on the promoter of p16^INK4a is investigated by using luciferase reporter plasmid pGL3-Basic-pl6N which has transcriptional regulation region between- 967 -- 165 of p16^INK4a promoter. The result shows that ZLJ increases the activity of p16^INK4a Dromoter. This may be the molecular mechanism by which ZLJ regulates the expression of P16 and inhibits the proliferation of BGC-823 cells.

关 键 词：紫龙金 p16^INK4a启动子 荧光素酶 

分 类 号：Q253[生物学—细胞生物学] S532[农业科学—作物学]