谷氨酰胺对急进高原大鼠肠道微生态影响的实验研究  

Effect of glutamine on change of intestinal microecology in rats exposed to acute high altitude

作　　者：张方信[1] 杨文翠[1] 邓芝云[2] 吴文明[1] 吴汉平[1] 陈嘉屿[1] 汪泳[1] 杨玉捷[1] 

机构地区：[1]兰州军区总医院消化科,甘肃兰州730050 [2]兰州军区总医院检验科,甘肃兰州730050

出　　处：《中国微生态学杂志》2010年第1期1-4,共4页Chinese Journal of Microecology

基　　金：全军"十一五"课题项目(06MA089)

摘　　要：目的探讨谷氨酰胺对急进高原大鼠小肠黏膜形态结构及肠道微生态的影响。方法W istar大鼠50只,随机分为5组:对照组(A组)、3848米未干预组(B组)、3848米谷氨酰胺干预组(C组)、4767米未干预组(D组)和4767米谷氨酰胺干预组(E组),每组10只,急进海拔3848米和4767米造成大鼠急性缺氧模型,检测小肠黏膜上皮细胞形态结构、肠道菌群失衡及细菌易位的变化。结果高海拔缺氧组大鼠小肠黏膜变薄、肠黏膜水肿、绒毛短缩,肠道菌群失衡显著高于对照组(P<0.05),且随着海拔升高,菌群失衡更明显。不同海拔高度细菌易位率也有差异。经谷氨酰胺干预后,肠道的菌群失衡及细菌易位率与高海拔缺氧组比较差异有显著性(P<0.05)。结论急进高原缺氧环境可导致小肠黏膜损伤、肠道菌群失衡及细菌易位,肠黏膜屏障破坏,且随着海拔升高而上述改变更明显。谷氨酰胺具有保护肠黏膜屏障及调节肠道菌群失衡的作用。Objective To observe the effect of glutaminc （GLN） on the morphosis and microecological change of intestinal mucosa in rats exposed to acute high altitude. Method 50 Wistar rats were randomly divided into control （A） group, altitude 3848 meters （B） group, altitude 3848 meters with glutamine intervention （C） group, altitude 4767 meters （D） group and altitude 4767 meters with glutamine intervention （E） group. 10 rats in each group. All rats except those in A group were posed acute hypoxia model at radical altitudes of 3848 meters and 4767 meters respectively. After 3 days of in- tervention with GLN or saline changes in the small intestinal epithelial cell morphology, intestnal flora imbalance and bacte- rial translocation were determined. Result After exposed to high attitude of hypoxia, the intestinal endothelial cells showed a series of pathological injuries including mucosal thinning, hydropsia, and villus shortening, etc. The intestinal flora im- balance were significantly higher （P 〈 0.05 ）. And as the altitude increased, the flora imbalance got more pronounced. The bacterial translocation rate differed in different altitudes. After the interveution by L-glutamine ,the intestinal flora imbalance and bacterial translocation rate were lower （ P 〈 0.05 ）. Conclusion Exposure to radical high altitude can influence the mucosa structure of small intestine, imbalance intestinal flora and bacterial translocation, and finally results in damage of in- testinal mucosal barrier. These changes were more evident with increasing elevation. Glutamine can play a role in protecting the mucosal barrier and regulating the intestinal flora imbalance.

关 键 词：谷氨酰胺 急进高原 肠道微生态 细菌易位 

分 类 号：R378[医药卫生—病原生物学]