三七凝胶对硬膜外粘连中COX-2、TGF-β_1的影响  

Effects of Sanqi Gel on expressions of cyclooxygenase-2 and transforming growth factor-β_1 during epidural adhesion

作　　者：王荣国[1] 周卫[2] 章永东[2] 孔焕宇[2] 李莉[2] 林新晓[2] 张万强[2] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]中国中医科学院望京医院

出　　处：《北京中医药大学学报》2010年第1期54-56,I0004,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的探讨三七凝胶预防硬膜外粘连(Epidural adhesion)的相关机制。方法将72只雄性SD大鼠随机分为生理盐水组、粘连平组、凝胶组和三七凝胶组,每组18只,建立椎板切除术模型,分别在术后第7、14、21天采用免疫组化进行动态观察,分析愈合过程中TGF-β_1和COX-2的表达情况。结果不同处理方法对TGF-β_1和COX-2的影响作用不同,作用强度存在差异。其中术后14 d生理盐水组、凝胶组和粘连平组COX-2的表达显著高于三七凝胶组(P<0.05);术后7、14、21 d 3个时间点中生理盐水组、凝胶组和粘连平组TGF-β_1的表达均显著高于三七凝胶组(P<0.05)。结论三七凝胶可能通过抑制COX-2和TGF-β_1的表达预防硬膜外瘢痕粘连。Objective To discuss the relevant mechanism of Sanqi Gel in the prevention of epidural adhesion. Method All male SD rats （n = 72） were randomly divided into the normal saline water group, Zhanlianping group, carbopol gel group and Sanqi Gel group （each n = 18 ）. The model of laminectomy was established and observed at different time points after the laminectomy for 7, 14 and 21 days respectively by applying immunohistochemistry method. The expressions of transforming growth factor-β1 （TGF-β1） and cyclooxygenase-2 （COX-2） were analysed during the healing period. Result The different treating methods had different effects on the expressions of TGF-β1 and COX-2 with different effective strength. The expression of COX-2 was higher significantly in the normal saline water group, carbopol gel group and Zhanlianping group than that in the Sanqi Gel group （P 〈0.05） 14 days after the operation. The expression of TGF-β1 was higher significantly in the normal saline water group, carbopol gel group and Zhanlianping group than that in the Sanqi Gel group （P 〈 0.05 ） at 3 time points （7, 14 and 21 days after the operation ）. Conclusion Sanqi Gel can prevent epidural adhesion through inhibiting the expressions of COX-2 and TGF-β1.

关 键 词：三七凝胶 粘连 椎板切除术 COX-2 TGF-Β1 大鼠 

分 类 号：R285.5[医药卫生—中药学]