利用bar基因导入恢复系提高杂交稻纯度的尝试  

Tests on Improving Purity in Hybrid Rice Seed Production by Introducing bar Gene to Restorers

作　　者：章善庆[1] 童汉华[1] 薛锐[1] 华志华[1] 汪晓玲[1] 黄大年[1] 谢小波 

机构地区：[1]中国水稻研究所

出　　处：《中国农业科学》1998年第6期33-37,共5页Scientia Agricultura Sinica

基　　金：中国农业科学院院长基金;浙江省"九五"生物技术重点资助

摘　　要：利用基因枪轰击水稻京引１１９未成熟胚获得转基因水稻植株Ｂ－１１９，经连续自交至Ｔ５代仍可稳定保持对Ｂａｓｔａ的抗性。采用人工杂交、回交转育等途径也可将转基因植株的ｂａｒ基因导入杂交水稻恢复系。使用ｂａｒ基因的恢复系所配制的杂交稻组合，秧苗期喷施０．２５％Ｂａｓｔａ药剂，可以有效清除混杂在杂交稻中的假杂交种，从而保证大田生产的纯度。研究结果对于拓宽杂交稻特别是两系杂交稻不育系的应用范围，展现出广泛的应用前景。ar gene was introduced to immature embroys of a japonica rice cultivar Jingying119 through biolistic system and a transgenic plant B 119 expressing resistance to Basta was obtained. The resistance to herbicide was stably maintained in five consecutive self crossing generations . It is simple and feasible to introduce the bar gene to restorer lines through intercross and backcross. Spraying of 0.25% Basta to hybrid rice seedling, the fake hybrid seedlings were killed by Basta and excluded from population for hybrid seed production to increase hybrid seed purity. It is helpful to clear up unstable fertility when widen the use of sterile lines.

关 键 词：抗除草剂 杂交水稻 纯度 转基因植株 

分 类 号：S511.035[农业科学—作物学]