一种与光镊结合的显微拉曼光谱技术  

A Micro-Raman Spectroscopy Combing with Optical Tweezers

作　　者：张勇[1] 叶安培[1,2,3] 闻丞[1] 

机构地区：[1]北京大学信息科学与技术学院,北京100871 [2]北京大学纳米器件物理与化学教育部重点实验室,北京100871 [3]北京大学前沿交叉学科研究院,北京100871

出　　处：《光学学报》2010年第2期491-497,共7页Acta Optica Sinica

基　　金：国家自然科学基金(10674008)资助课题

摘　　要：论述了一种与光镊结合的显微拉曼光谱即"光镊-显微拉曼光谱"系统的构建方案。给出了利用该系统测得的活细胞(大白鼠血细胞,酵母细胞)的微区拉曼光谱,并将红外光镊束缚下的Ar+激光激发的血细胞的拉曼光谱,与用单一Ar+激光光镊束缚并同时激发的血细胞拉曼光谱,以及血细胞直接贴在基底上的拉曼光谱进行了分析对比。实验表明,前者比后两种实验方案的血细胞拉曼光谱信号有显著提高。分析了红外1064 nm光镊激光和可见514.5 nm Ar+激光对活细胞的损伤效应,发现高功率的红外激光对细胞的损伤作用远小于低功率的Ar+激光。并将该系统应用于碳纳米管材料分析,获得了单壁碳纳米管的拉曼光谱。A hybrid ＂optical tweezers-micro Raman spectroscopy＂ technique is introduced in this letter. Applying this system,the micro-Raman spectrums of rat erythrocyte and saccharomyces cerevisiae in vivo were obtained. The results show that this technique can obviously improve signal-to-noise ratio compared with conventional approaches. Besides,the damage from lasers on the cells were analyzed. It was revealed that the damage of cell from intense IR laser were much lower than that from the weak Ar^＋ laser. Finally,the system was also applied in carbon nano-tube （CNT） analysis and Raman spectra from CNT were obtained.

关 键 词：显微拉曼光谱 激光光镊 光镊-拉曼光谱系统 活细胞拉曼光谱 单壁碳纳米管拉曼光谱 

分 类 号：O437.3[机械工程—光学工程]