PEG介导BT基因转化大豆原生质体获转基因植株  

PEG-MEDIATED TRANSFORMATION AND REGENERATION OF SOYBEAN PROTOPLAST WITH BACILLUS THURINGIENSIS CrgIAc GENE

作　　者：南相日[1] 刘文萍[1] 刘丽艳[1] 吕晓波[1] 何云霞[1] 卫志明[2] 

机构地区：[1]黑龙江省农业科学院生物技术研究中心 [2]中国科学院上海植物生理研究所

出　　处：《大豆科学》1998年第4期326-330,共5页Soybean Science

基　　金：中国科学院上海植生所植物分子遗传国家重点实验室资助

摘　　要：通过ＰＥＧ法将Ｂ．Ｔ（ＢａｃｉｌａｓｔｈａｒｉｎｇｉｅｎｓｉｓＣｒｙＩＡｃ）毒蛋白基因导入到大豆主栽品种黑农３５，黑农３７，合丰２５和合丰３５的原生质体中，经３０ｍｌ／Ｌ潮霉素筛选，选择有抗性的愈伤组织进行分化，获得了三棵再生植株，移栽后全部成活，对移栽后植株的总ＤＮＡ进行ＰＣＲ分析，均显阳性。对ＰＣＲ阳性植株进行Ｓｏｕｔｈｅｒｎ杂交分析，证明Ｂ．Ｔ毒蛋白基因已整合到大豆细胞基因组中。acillus thuringiensis CryIAc gene was introduced into the protoplast of leading soybean cultivars Heinong35, Heinong37, Hefeng25 and Hefeng35 with the PEG method. Through the screening with 30mg/l hygromycin and the differentiation of selected resistant calli, three regenerated plants were obtained and transplanted successfully. PCR analysis of the DNA from the transplanted plants showed positive reaction. Southern blot analysis of the PCR-positive plants proved that the B.T CryIAc gene had integrated into the genome of these plants.

关 键 词：大豆 原生质体 B.T毒蛋白基因 转基因植株 

分 类 号：S565.103.2[农业科学—作物学]