梅毒检测“灰区”样本的分析  

Analyses of syphilis assay for the samples in the gray zone

作　　者：曹树正[1] 王文武[1] 张真路[1] 

机构地区：[1]武汉亚洲心脏病医院检验中心,430022

出　　处：《实用医学杂志》2010年第3期476-477,共2页The Journal of Practical Medicine

摘　　要：目的:通过分析ELISA检测处于"灰区"处的样本,探讨适宜的梅毒检测操作规程,减少漏检。方法:用梅毒酶联免疫吸附法(TP-ELISA)对13802份血清样本进行初筛,对处于灰区的样本,再进行甲苯胺红不加热血清试验和梅毒螺旋体明胶凝集试验(TPPA)进行确认。结果:共有15例处于灰区的样本,其中有5例经TPPA确证试验为阳性。结论:TP-ELISA检测仍存在一定的假阴性,设定合理的灰区范围,对灰区内的样本进行确认试验对减少漏检具有重要的临床意义。Objective To eliminate the omission and to get eligible syphilis test operating procedure through analysing the samples in the gray zone in ELISA test. Methods 13 802 serum samples were screened by TP-ELISA assay, and those in the gray zone were confirmed by toluidine red unheated-serum test （TRUST） and treponema pallidum particle agglutination test （TPPA） assay. Result There were 15 samples in the gray zone, among which 5 samples were confirmed positive by TPPA assay. Conclusion There is a little false negative rate in TP-ELISA test, in case of which setting the gray zone range and using the confirmatory test to eliminate omission have considerable clinical significance.

关 键 词：梅毒螺旋体 “灰区” 酶联免疫吸附试验 甲苯胺红不加热血清试验 梅毒螺旋体明胶凝集试验 

分 类 号：R446.6[医药卫生—诊断学] R759.1[医药卫生—临床医学]