HPLC法测定九香止泻片中秦皮甲素和秦皮乙素的含量  

Determination of Aesculin and Aesculetin in Jiuxiang Zhixie Tablets by HPLC

作　　者：赵碧清[1] 何群[1] 滕久祥[1] 彭芝配[1] 李慧[1] 王适[1] 

机构地区：[1]湖南中医药大学药学院,长沙市410208

出　　处：《中国药房》2010年第7期626-628,共3页China Pharmacy

基　　金：湖南省教育厅科学研究项目(05A0312)

摘　　要：目的:建立九香止泻片中秦皮甲素和秦皮乙素的含量测定方法。方法:采用高效液相色谱法,色谱柱为Thermo C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(12∶88),流速为1.0mL·min-1,检测波长为334nm,柱温为30℃。结果:秦皮甲素进样量在80～800ng范围内与峰面积积分值呈良好线性关系(r=0.9998),平均回收率为100.1%,RSD=1.89%(n=9);秦皮乙素进样量在32.96～329.6ng范围内与峰面积积分值呈良好线性关系(r=0.9995),平均回收率为101.5%,RSD=2.42%(n=9)。结论:本法简单易行,结果准确可靠,可作为九香止泻片的含量测定方法。OBJECTIVE： To establish a method for the determination of aesculin and aesculetin in Jiuxiang zhixie tablets. METHODS： HPLC method was developed to quantitative determination. Thermo C18（250 mm×4.60 mm, 5μm） column was adopted. The mobile phase consisted of acetonitrile-0.1% H3PO4 （12：88）with flow rate of 1.0 mL·min-1 and the detection wavelength of 334 nm. The column tempreture was set at 30℃. RESULTS： The linear range of aesculin was 80～800 ng（r=0.999 8）.The average recovery was 100.1% （RSD=1.89% ,n=9）. The linear range of aesculetin was 32.96～329.6 ng（r=0.999 5）. The average recovery of aesculetin was 101.5% （RSD=2.42% ,n=9）. CONCLUSION： The method is simple, accurate for the content determination of Jiuxiang zhixie tablet.

关 键 词：高效液相色谱法 九香止泻片 含量测定 秦皮甲素 秦皮乙素 

分 类 号：R283.64[医药卫生—中药学] R927.2[医药卫生—中医学]