诱导固氮菌与稻苗结瘤共生的研究  

Inducing nodulation on rice by nitrogen fixing bacteria

作　　者：潘建菁[1,2] 陈启锋[1,2] 黄世贞[1,2] 

机构地区：[1]福建农业大学作物科学学院 [2]福建农科院土肥所

出　　处：《福建农业大学学报》1998年第4期405-410,共6页Journal of Fujian Agricultural University

摘　　要：以植物生长激素２，４－Ｄ处理非豆科植物稻苗并接种固氮菌，诱导固氮菌与幼苗结瘤（类瘤）共生．多次重复实验结果如下：不同稻品种的始瘤日期、结瘤率及固氮酶活性有所差异．在１２个供试品种中，始瘤期需３～１２ｄ；结瘤率为２０．５％～１００％；固氮酶活性（乙炔还原法）最高的达７７．６ｎｍｏｌ株－１ｄ－１．稻苗经２，４－Ｄ处理后接种弗兰克氏菌，取得与根瘤菌相似结果．这是弗兰克氏菌应用于农作物的首次报道．固氮菌与稻苗共生固氮有促进生长、延缓衰老的作用．本研究揭示了根瘤菌与禾本科作物之间的一种新的共生关系，对研究非豆科作物结瘤固氮将是很有意义的．Nitrogen fixing bacteria were introduced into rice roots for nodulation(para nodules) and symbiosis by the addition of synthetic auxin 2,4 D.The results of repeated experiments were as follows.The dates of the first nodule appearance , nodulation rates and nitrogenase activities (Acetylene Reduction Assay) were different. In 12 tested rice varieties, the first nodule like structures need 3 to 12 days to appear, the nodulation rate varied from 20.5% to 100% and the maximum nitrogenase activity reached 77. 6 nmol per plant one day. The results from treatment with 2,4 D and inoculation with Frankia were similar to those from treatment with 2,4 D and Rhizobium , which was the first report.The symbiosis between nitrogen fixing bacteria and rice seedlings could promote growth. In this study, the symbiotic relationship between Rhizobium and cereal crops was revealed.It was important for the research on nodulation and nitrogen fixation of non legume crops.

关 键 词：稻 根瘤菌 弗兰克氏菌 诱导结瘤 共生固氮 

分 类 号：S511[农业科学—作物学] S144.3