Catalytic Characteristics of Lipase Immobilized in CTAB/TX-100 MBG  

作　　者：SONG Shao-fang LUAN Yu-xia 

机构地区：[1]College of Chemistry and Material Science, Shandong Agricultural University, Tai ＇an 271018, P. R. China [2]Institute of Pharmaceuties, School of Pharmaceutical Science, Shandong University, Jinan 250100, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第1期110-113,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No. 20803044);the Foundation of Key Laboratory of Colloid and Interface Chemistry of Ministry of Education,Shandong University,China(No.200714)

摘　　要：CTAB/TX-100 microemtdsion-based gel（MBG）, which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.CTAB/TX-100 microemtdsion-based gel（MBG）, which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.

关 键 词：Microemulsion-based gel（MBG） Immobilized lipase Activity STEREOSELECTIVITY ESTERIFICATION Hydrolysis 

分 类 号：O643.32[理学—物理化学] TQ423.2[理学—化学]