鲍曼不动杆菌Ⅰ类整合子与多重耐药相关性研究  

Study on the relationship between class Ⅰ integron and multidrug resistance in Acinetobacter baumannii clinical isolates

作　　者：林丽[1] 凌保东[1] 张翔[1] 袁斌[1] 

机构地区：[1]川北医学院药物研究所,南充637007

出　　处：《中国抗生素杂志》2010年第1期54-58,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(30973662)

摘　　要：目的了解临床分离鲍曼不动杆菌的耐药状况、Ⅰ类整合子的分布情况,探讨Ⅰ类整合子与多重耐药的关系。方法检测20种临床常用抗菌药物对鲍曼不动杆菌临床分离株的最低抑菌浓度(MIC)。PCR扩增Ⅰ类整合酶基因。对部分Ⅰ类整合酶阳性菌株进行耐药基因盒序列分析。结果鲍曼不动杆菌呈现多重耐药,鲍曼不动杆菌对IMP和MRP耐药率分别为0.9%和1.8%,对CPZ/SB的耐药率为35.7%,对其它抗菌药物的耐药率均大于60%,多重耐药率为76.8%(86/112),但对COL和MIN均敏感。80.4%(90/112)的菌株检测出Ⅰ类整合子。Ⅰ类整合子阳性株对多种药物的耐药率均高于阴性株,且Ⅰ类整合子阳性株多重耐药率(90%)明显高于阴性株(22.7%)(P<0.01)。Ⅰ类整合子基因盒序列分析显示,Ⅰ类整合子携带aacA4,catB8和aadA13种耐药基因。结论Ⅰ类整合子在鲍曼不动杆菌中检出率很高并与其多重耐药性关系密切。Objective To investigate the distribution and characterization of the class I integron genes （intI1） and integrated gene cassettes in Acinetobacter baumannii isolates and analyze the relationship between class I integron and multidrug resistance. Methods Antimicrobial susceptibility testing was determined by serial two-fold dilution. Class I integrons were detected by polymerase chain reaction in 112 Acinetobacter baumannii isolates. The integrated gene cassettes were determined by DNA sequencing. Results Most isoliates tested were multidrug resistance, intI1 was detected in 80.4%（90/112） of the isolates. For most antibacterials tested, the resistant rates of integron-positive strains were higher than integron-negative strains. The incidence of multidrug resistant isolates in integron-positive strains（90%） was much higher than those of integron-negative strains（22.7%）（P〈0.01）. The sequencing of integrated gene cassettes showed the class I integron habouring the genes of aacA4, aadA1 and catBS, which confer aminoglycoside and chloramphenicol resistance respectively. Conclusions High incidence of class I integrons was found in Acinetobacter baumannii isolates and close association existed between class I integrons and multiple drug resistance.

关 键 词：鲍曼不动杆菌 多重耐药 整合子 整合酶 

分 类 号：R378.2[医药卫生—病原生物学]