西洋参发根的诱导及其培养条件的研究  

Induction of Hairy Roots of Panax quinquefolium by Ri Plasmid and Research on Suitable Culture Condition of Panax quinquefolium Hairy Roots

作　　者：贾冬梅[1] 赵寿经[2] 钱延春[2] 吴颖[1] 杨柏明[1] 张博[3] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]吉林大学生物与农业工程学院,长春130022 [3]吉林大学生命科学学院,长春130000

出　　处：《吉林农业大学学报》2010年第1期58-61,共4页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20060546);吉林大学农学部青年基金项目(200826)

摘　　要：利用发根农杆菌A4菌株在西洋参根外植体上直接诱导产生发根。在1/2 MS和MS固体培养基上得到西洋参发根,并在B5液体培养基上建立起发根离体培养系,经连续多代的培养,发根仍保持旺盛生长状态。PCR扩增结果表明,发根农杆菌Ri质粒的rolC基因已整合到西洋参发根基因组中并得到表达。利用紫外分光光度法测得西洋参发根的总皂苷含量达3.88%。经对发根比生长速率的测定,确定1/2 B5培养基(15 g/L蔗糖)、摇床转速100 r/min、每4周更换1次培养基并继代培养1次为西洋参发根生长适宜条件。Hairy roots of Panax quinquefolium L. were induced from the root explants of two-year old Panax quinquefolium L. by Agrobacterium rhizogenes A4 with direct inoculation, the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums. After several times of subcultures, the hairy roots still maintained vigorous growth. After the determination of UV-spectrophotometry, the total ginsenosides content in the hairy root came up to 3.88%. The suitable culture conditions for Panax quinquefolium L. hairy roots growing were 1/2 B5 liquid medium（ 15 g/L glucose）, in a shaker at 100 r/min, changing the culture solution and subeuhuring the hairy roots every. 4 weeks.

关 键 词：生物工程 发根 农杆菌 西洋参 

分 类 号：S567.53[农业科学—中草药栽培]