重型肝炎患者肝组织中增殖细胞核抗原的检测  

Detection for PCNA in liver tissue with severe hepatitis

作　　者：郎振为[1] 李德芬[1] 金荣华[1] 李俊强[1] 

机构地区：[1]北京佑安医院,北京市100054

出　　处：《胃肠病学和肝病学杂志》1998年第4期339-342,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的:观察重型肝炎时肝细胞的再生状况.方法:应用免疫组化法对48例重型肝炎肝组织中的增殖细胞核抗原(PCNA)进行了检测.结果:急性重型和亚急性重型肝炎肝组织中的PCNA阳性细胞明显高于慢性重型肝炎(P<O.01),生存者与死亡者相比,其肝组织中的PCNA标记指数有极明显差别(P<O.01).结论:在急性和亚急性重型肝炎,肝组织内肝细胞较大范围坏死后,残留的肝细胞仍能进行再生,肝组织中PCNA的检测对于判断重型肝炎患者的预后是一种有用的指标.Aims: To observe the regeneration of hepatocytes in severe hepatitis. Methods: Liver samples from 48 patients with severe hepatitis were detected for proliferating cell nuclear antigen (PCNA) by immunohistochemical methods.Results: The labelling index (L. I. ) of PCNA-positive hepatocytes was higher in the patients with acute severe hepatitis and subacute hepatitis than in the patients with chronic severe hepatitis ( P< 0.01) . PCNA positivity was significantly higher in the patients who survived than in the patients who died (P<0.01) .Conclusion; Hepatocyte regeneration could take place in severe hepatitis , including acute severe hepatitis, after development of massive necrosis of liver cells and detection for PCNA in liver tissue could be useful for prognostic evaluation.

关 键 词：免疫组化 增殖细胞核抗原 重型 肝炎 预后 

分 类 号：R512.607[医药卫生—内科学]