三七总皂苷对脑缺血再灌注损伤大鼠神经干细胞相关调节因子及脑细胞凋亡的影响  

Effects of Total Panax Notoginseng Sapoinis on Brain Cell Apoptosis and Regulatory Factor in Neural Stem Cell in Rats with Cerebral Ischemia Reperfusion Injury

作　　者：钟森[1] 陈文超[2] 徐永强[1] 黄永章[2] 杨建业[2] 张晓燕[2] 

机构地区：[1]湖北省十堰市妇幼保健院,十堰442000 [2]郧阳医学院附属人民医院,十堰442000

出　　处：《中国中医急症》2010年第2期279-282,共4页Journal of Emergency in Traditional Chinese Medicine

摘　　要：目的观察三七总皂苷(PNS)对脑缺血再灌注损伤(CIRI)脑组织细胞抑凋亡基因B细胞淋巴瘤/白血病-2(Bcl-2)及中枢神经系统神经干细胞(NSCs)相关调节因子巢蛋白(Nestin)、脑源性神经营养因子(BDNF)、表皮生长因子(EGF)的影响,并探讨其相互关系。方法大鼠随机分为假手术组、模型组、PNS组3组;采用线栓法制作大鼠局灶性脑缺血再灌注模型;缺血30min后腹腔注射三七总皂苷,再灌注1h、3h、5h后取组织标本;免疫组织化学法检测Bcl-2、Nestin、BDNF、EGF蛋白的表达。结果缺血再灌注各时间点,PNS组Bcl-2、Nestin、BDNF、EGF的表达与模型组比较明显增加,与假手术组比较增加显著,模型组Bcl-2、Nestin、BDNF、EGF的表达较PNS组与假手术组明显减少。结论三七总皂苷通过上调Bcl-2的表达而抑制神经细胞凋亡,同时增强Nestin、BDNF、EGF蛋白的表达而促进NSCs增殖,起到抗CIRI的作用。Objective： To investigate the effects of the panax notoginseng saponins （PNS） on brain cell apoptosis inhibitory gene B -cell lymphoma/teukemia- 2（Bcl -2）, Nestin, brain- derived neurotrophie factor（BDNF）, epi- dermal growth factor（EGF） of rats with cerebral ischemia- reperfusion injury. Methods： Rats established by suture method were randomly divided into the sham operation group, the model group and the PNS group. In ischemia 30min, the PNS was injected into the peritoneal. After reperfusion lh, 3h and 5h, tissue samples were taken from these models. And then, Bcl -2, Nestin, BDNF and EGF were detected by inmunohistochemistry method. Results： At dif- ferent time points of ischemia - reperfusion, these expressions in the model group were not only increased more sig- nificantly those in the model group, but also added more than those in the sham operation group. These indices in the model group were decreased remarkably compared with the PNS group and the sham operated group. Conclusion： In order to resistant the CIRI effect, the PNS could promote the NSCs proliferation. This condition is realized by up - regulating the expression of Bcl - 2 which is to inhibit the neuapoptosisronal and enhance the Nestin, BDNF, EGF protein expressions .

关 键 词：脑缺血再灌注损伤 神经干细胞 凋亡B细胞淋巴瘤/白血病-2 巢蛋白 脑源性神经营养因子 表皮生长因子 三七总皂苷 

分 类 号：R285.5[医药卫生—中药学]