活性氧在p,p’-DDE诱导大鼠睾丸支持细胞凋亡中的作用  

The Role of Reactive Oxygen Species in p,p'-DDE-induced Apoptosis of Sertoli Cells in Rats

作　　者：宋杨[1] 石玉琴[1] 程晋[1] 关霞[1] 王玉萍[1] 杨克敌[1] 

机构地区：[1]华中科技大学同济医学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,湖北武汉430030

出　　处：《环境与职业医学》2010年第1期24-27,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:30671734)

摘　　要：[目的]研究活性氧在2,2-双-(对氯苯基)-1,1-二氯乙稀(p,p’-DDE)诱导大鼠睾丸支持细胞凋亡中的作用。[方法]从大鼠睾丸组织中分离支持细胞进行离体原代培养3d,用0、10、30、50μmol/L的p,p’-DDE及加有300μmol/L的抗氧化剂N-乙酰-L-半胱氨酸(NAC)的p,p’-DDE(50μmol/L)继续培养24h,应用流式细胞仪测定细胞内活性氧(ROS)水平、线粒体膜势能(△ψm)、凋亡发生率。[结果]50μmol/L的p,p’-DDE可以诱导支持细胞ROS显著升高,10、30、50μmol/Lp,p’-DDE处理组的线粒体膜势能下降,30、50μmol/L的p,p’-DDE可以诱导支持细胞凋亡,与对照组相比,差异有统计学意义(P<0.05);NAC有效抑制了ROS升高,削弱线粒体膜势能下降,减少了凋亡发生率,与50μmol/L的p,p’-DDE处理组相比,差异有显著意义(P<0.05)。[结论]p,p’-DDE可以诱导大鼠睾丸支持细胞凋亡,ROS产生可能是其重要原因之一。[ Objective ] To investigate the mechanism of p,p＇-DDE-induced apoptosis of Sertoli cells and the role of reactive oxygen species. [ Methods ] Sertoli cells from testes of rats were treated with 0, 10, 30, and 50 μmol/L p,p＇-DDE, or with 50 μmol/L p,p＇-DDE pretreated by 300 μmolFL NAC （ N-acetyl-L-cysteine ）. Then the fluoresence intensity of reactive oxygen species（ ROS ）, mitochondria membrane potential（ △ψm ）and apoptosis percentage was detected by a FACS Calibur flow cytometer. [ Results ] 50 μmol/L p,p＇-DDE increased the ROS level. The mitochondria membrane potential was decreased after all p,p＇- DDE＇s treatments. The percentage of apoptosis was increased after treatment with 30 or 50 μmol/L p,p＇-DDE. Compared with the control group, the difference was statistically significant（ P 〈 0.05 ）. The antioxidant NAC inhibited the increased ROS generation, decreased the mitochondria membrane potential and apoptosis percentage （ P 〈 0.05 ）. [ Conclusion ] p,p＇-DDE could induce apoptosis in Sertoli cells. The generation of ROS may be an important reason in the apoptosis.

关 键 词：2 2-双-对氯苯基-1 1-二氯乙稀 活性氧 睾丸支持细胞 凋亡 

分 类 号：R114[医药卫生—卫生毒理学]