鸦胆子不同组分对小鼠急性毒性的比较研究  

Comparative Study on Acute Toxicity of Different Components of Fructus Bruceae in Mice

作　　者：孙蓉[1] 杨倩[2] 张作平 吕莉莉[2] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355 [3]济南杏林生物技术有限公司,山东济南250101

出　　处：《中国药物警戒》2010年第2期73-77,共5页Chinese Journal of Pharmacovigilance

基　　金：国家973计划中医基础理论专项;确有疗效的有毒中药科学应用关键问题的基础研究(项目编号:2009CB522800);有毒中药的毒性与功效;证候关系的基础研究(课题编号:2009CB522802)

摘　　要：目的比较鸦胆子全组分、水提组分、醇提组分对小鼠的急性毒性反应。方法采用经典的急性毒性实验方法测定鸦胆子全组分、水提组分、醇提组分的小鼠口服半数致死量,观察其急性毒性症状谱,记录累积死亡数及小鼠体重变化。结果鸦胆子全组分、水提组分、醇提组分的小鼠口服LD50分别为3.14g·kg-1、4.023g·kg-1、3.320g·kg-1,分别相当于临床日用量的110倍、140.8倍、116倍。腹泻、尾部紫绀是主要的急性毒性症状。结论鸦胆子不同组分急性毒性大小顺序依次是全组分、水提组分、醇提组分,其毒性成分与中毒机理尚待进一步研究。Objective Compare the acute toxicity of different components of Fructus Bmceae. Methods Ig all-components,water extract and alcohol extract of Fructus Brucea to mice and test the LD50 of oral dose in mice by the classic methods of acute toxicity. The acute toxic symptoms were observed; the accumulated death number and the weight-changes of live mice were recorded. Results The LD50 of all-components ,water extract and alcohol extract of Fructus Brucea to mice are 3.14g·kg^-1,4.023g·kg^-1,3.320g·kg^-1.They are respectively equal to 110 ,140.8,116 times of the daily dosage in clinical. Diarrhea and cyanosis are main acute toxic symptoms. Conclusion The size order of acute toxicity of different components in Fructus Brucea is all-components 〉alcohol extract〉water extract. The toxical components and toxical mechanism should be researched furtherly.

关 键 词：鸦胆子 全组分 水提组分 醇提组分 急性毒性 

分 类 号：R282[医药卫生—中药学]