阿是止泻胶囊对甲状腺功能亢进性腹泻大鼠胃窦、结肠黏膜肥大细胞的影响  

Effect of Asi-antidiarrheal capsule on mast cell in sinus ventriculi and colon mucosa of rats with thyroid hormone-induced diarrhea

作　　者：毕长龙[1] 张瑛琦[1] 翟爱霞[2] 房泽慧[1] 郭敏[1] 成志锋[1] 

机构地区：[1]哈尔滨医科大学附属第四医院内分泌科,150001 [2]哈尔滨医科大学微生物学教研室

出　　处：《国际免疫学杂志》2010年第1期65-68,共4页International Journal of Immunology

基　　金：黑龙江省科委“十五”攻关基金资助项目（GCO1C138201）

摘　　要：目的探讨阿是止泻胶囊对甲状腺功能亢进（简称甲亢）性腹泻大鼠胃窦、结肠黏膜肥大细胞（MC）的影响。方法制备甲亢性腹泻大鼠模型。运用阿是止泻胶囊低、中、高剂量及黄连素对其干预．采用免疫组化技术测定对照组、模型组、黄连素组、阿是止泻胶囊低、中、高剂量组大鼠胃窦、结肠黏膜MC数量、面积、周长、等效直径及圆形度：结果①与对照组比较，模型组胃窦黏膜ME数目（P=0．003）、脱颗粒比率（P=0．029）、面积（P=0．000）、周长（P=0．000）、等效直径（P：0．000）增大，网形度变小（P=0．000）、：②与模型组比较，黄连素组、高剂量组胃窦黏膜MC数目（P=0．003，P=0．003）、面积（P=0．000，P=0．000）、周长（P=0．000，P=0．000）、等效直径（P=0．000，P：0．000）变小，圆形度增大（P=0．000，P=0．000）。③胃窦黏膜各组间MC数目（F=3．462，P=0．014）、面积（F=37．632，P=0．000）、周长（F=47．993，P=0．000）、等效直径（F=60．970，P=0．000）、圆形度（F=15．642，P=0．000）比较，差异均有统计学意义。④结肠黏膜各组间MC数目差异无统计学意义（F=0．82，P=0．546）。结论阿是止泻胶囊能显著降低胃窦黏膜MC数目，可能是其治疗甲亢性腹泻的机制。Objective To investigate the Asi-antidiarrheal capsule＇s effect on mast ceils （MC） in sinus ventriculi and colon mucosa of rats with thyroid hormone-induced diarrhea （HD）. Methods Thyroid hormone-induced diarrheic rats were prepared and interfered by low, medium, and high doses of Asi-antidiarrheal capsule, respectively. Immunohistochemistry was used to detect the population, area, perimeter, equivalent diameter, and degree of circularity of the MC in sinus ventriculi and colon mucosa of control group （CG） , model group （MG）, berberine group （BG）, and Asi-antidiarrheal capsule group （AG）. Results Compared with CG, the population （ P = 0. 003 ）, ratio of degranulation （ P = 0. 029 ）, area （ P = 0. 000 ）, perimeter （P =0. 000） and equivalent diameter （P = 0. 000） of the MC in sinus ventriculi mucosa were increased and the degrce of circularity （P =0. 000） was decreased in MG. Compared with MG, the population （P =0. 003, P = 0. 003 ） , area （ P = 0. 000, P = 0. 000 ） , perimeter （ P = 0. 000, P = 0. 000 ）, and equivalent diameter （P =0. 000,P = 0. 000） of the MC in sinus ventriculi mucosa were decreased and the degrce of circularity （ P = 0. 000, P = 0. 000） was increased in BG and high dose AG. The population （ F = 3. 462, P = 0. 014 ） ,area （F = 37. 632, P = 0. 000）, perimeter （ F = 47.993, P = 0. 000）, equivalent diameter （ F = 60. 970, P = 0. 000） and degree of circularity （ F = 15.642, P = 0. 000） of MC had statistical significance in each group of sinus ventriculi mucosa. The number of MC had no statistical significance in each group of colon mucosa （ F = 0. 82, P = 0. 546）. Conclusion Asi-antidiarrheal capsule can significantly decrease the number of MC in sinus ventriculi mucosa and may have potential effects on diarrhea of hyperthyroidism.

关 键 词：甲状腺功能亢进 腹泻 阿是止泻胶囊 肥大细胞 

分 类 号：R28[医药卫生—中药学]