伏牛白山羊6个微卫星标记的遗传多样性分析  

Genetic Polymorphisms of Six Microsatellite Markers in Funiu White Goat

作　　者：张娜娜[1] 王清义[2] 毛薇[2] 王玉琴[1] 王占彬[1] 张小辉[1] 白俊艳[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]郑州铁路职业技术学院,河南郑州450052

出　　处：《河南农业科学》2010年第2期92-96,107,共6页Journal of Henan Agricultural Sciences

基　　金：河南省重点科技攻关项目(072102130004);河南省自然科学基础研究计划项目(2008A23001)

摘　　要：选取位于绵羊第6号染色体上与牛多胎基因FecB紧密连锁的2个微卫星基因座OarAE1 01、BM1329,牛第3号染色体上的微卫星基因座BMS1248,绵羊第4号染色体上的2个微卫星基因座MAF70、OarHH35及牛第8号染色体上的微卫星基因座BM1227,对伏牛白山羊遗传多样性进行检测。结果表明,6个微卫星基因座在伏牛白山羊中共检测到50个等位基因,其中平均多态信息含量(PIC)为0.789,且每个微卫星基因座的PIC>0.5,平均有效等位基因数(Ne)、平均杂合度(H)分别为5.165、0.903。由此表明,6个微卫星标记均具有高度多态性,可用于伏牛白山羊遗传多样性的分析。To investigate the genetic diversity of microsatellite markers for Funiu white goat, the microsatellite loci of OarAE101 and BM1329 linked to the fecundity gene FecB mapped to chromosome 6 in sheep and cattle,BMS1248 located on chromosome 3 in cattle, MAF 70 and Oar- HH35 located on chromosome 4 in sheep and BM1227 located on chromosome 8 in cattle were analyzed. A total of 50 alleles were detected in Funiu white goat varieties. The average polymorphism information content（PIC）of 6 microsatellite loci was 0. 789,and the PIC of each microsatellite locus was above 0.5. The average number of effective alleles（Ne）and average heterozygosity（H）at 6 microsatellite locus was 5. 165 or 0. 903,respectively. These 6 microsatellite markers show rich polymorphisms for analyzing the genetic diversity of Funiu white goat.

关 键 词：伏牛白山羊 遗传多态性 微卫星标记 

分 类 号：S827[农业科学—畜牧学]