兔骨髓间充质干细胞的分离培养  

Preparation and Cultivation of Rabbit Bone Marrow-derived Mesenchymal Stem Cells

作　　者：闫颖颖[1,2] 张静芳[1] 和小娥[2] 王新庄[1,2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业科学》2010年第2期102-104,共3页Journal of Henan Agricultural Sciences

基　　金：国家"863"高新技术项目(2008AA101010);河南农业大学人才引进基金;河南省基础与前沿技术研究计划(092300410081)

摘　　要：为探讨体外分离及培养扩增兔骨髓间充质干细胞(MSCs)的方法,无菌采取兔股骨,用含20%FBS的DMEM培养液冲骨髓腔,采用全骨髓细胞贴壁培养法对MSCs进行纯化扩增,倒置显微镜下观察原代及传代细胞的形态、生长情况、绘制细胞生长曲线。结果显示,MSCs为贴壁生长细胞,形态均匀呈纤维样,增殖能力强,多次传代仍能保持其生物学特性。采用全骨髓贴壁培养法能够较好地纯化和扩增MSCs。In this study, an effective method to prepare and culture rabbit bone marrow derived mesenchymal stem cells （MSCs） was established. After the femurs were dissected from rabbit, the marrow was flushed out with DMEM medium under aseptic condition. The MSCs were en- riched and expanded by using bone marrow adherent culture, and inverted microscope was em- ployed to observe the proliferation and their morphous of primary and passage cells. The result shows that MSCs are adherent cells with a similar fihroblastoid spindle-shaped morphology during different passages. Bone marrow adherent culture can be used to produce a certain purity of MSCs, which is an easy way to ensure a good condition and powerful productivity for cell.

关 键 词：骨髓间充质干细胞 体外培养 兔 

分 类 号：Q813.1[生物学—生物工程]