反复热性惊厥大鼠海马IL-1β和IL—1ra表达的变化  

Changes of IL-1β and IL-1ra expression in the hippocampus of rats with frequent febrile seizures

作　　者：郑飞霞[1] 李光乾[1] 施旭来[1] 林忠东[1] 

机构地区：[1]温州医学院附属育英儿童医院神经内科,325027

出　　处：《中国小儿急救医学》2010年第1期37-39,共3页Chinese Pediatric Emergency Medicine

基　　金：温州市科技局科技发展计划项目（Y2004A012）

摘　　要：目的探讨大鼠反复热性惊厥（FS）后海马白细胞介素-1β（IL-1β）和白细胞介素-1受体拮抗剂（IL-1ra）表达的变化。方法清洁级21日龄雄性SD大鼠随机分为正常对照组（NC组）、高热对照组（HC组）和热性惊厥组（FS组）。末次热水浴后12h处死动物，用ELISA法检测海马IL-1β和IL-1ra的含量；RT—PCR法测海马IL-1β和IL-1ramRNA的表达水平。结果FS组大鼠海马IL-1β蛋白和IL-1βmRNA水平均明显高于NC组和HC组，差异均具有显著性（P〈0．01和P〈0．05）；FS组大鼠海马IL-1ra蛋白及IL—1ramRNA水平亦明显高于HC组和NC组，差异均具有显著性（P〈0．01和P〈0．05）。各组大鼠海马IL-1ra／IL-1β蛋白比值差异无显著性（P〉0．05）。结论短期内反复FS可导致海马IL-1β和IL-1ra表达增加，提示IL-1β和IL-1ra可能参与FS脑损伤发病的病理过程。Objective To evaluate the changes of IL-1β and IL-1 ra in hippocampus of rats with frequent febrile seizures（FS）. Methods 21-day-old male Sprague-Dawley （SD） rats were randomly divided into 3 groups ： normal control group （ NC group）, hyperthermic control group （ HC group） and febrile seizures group （FS group）. IL-1β and IL-1ra levels in hippocampus of these rats were determined by ELISA methods,IL-1β mRNA and IL-1ra mRNA levels in hippocampus were measured by RT-PCR. Results IL- 1β mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group（ P 〈 0. 01, P 〈 0. 05 ）. IL-1ra mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group（P 〈0. 01 ,P 〈0. 05） ,too. IL-1ra/IL- 1β protein ratio showed no significant difference among 3 groups. Conclusion Frequent FS can cause the in crease of IL-1β and IL-1ra mRNA and protein levels in hippocampus,suggesting that IL-1β and IL-1ra may be involved in the pathogenesis of hippocampal neuron damage in FS rat.

关 键 词：惊厥 发热性 白细胞介素-1Β 白细胞介素-1 受体拮拉剂 海马 大鼠 

分 类 号：R720.597[医药卫生—急诊医学] R742.1[医药卫生—儿科]