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机构地区:[1]青岛农业大学动物科技学院,山东青岛266109 [2]青岛澳兰百特生物工程有限公司,山东青岛266101
出 处:《动物医学进展》2010年第2期78-83,共6页Progress In Veterinary Medicine
摘 要:应用RT-PCR对山东青岛、潍坊和烟台3个地区2008年1月至2009年6月送检的1837份具有呼吸道症状鸡的病料进行新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)3种主要病毒感染情况的调查。调查显示,3个地区3种病毒的感染率可达65.76%,其中NDV、IBV和AIV(H9)的平均阳性率分别为12.36%、12.30%和41.10%。青岛地区NDV、AIV(H9)和IBV的阳性率最高,分别为13.45%、17.37%和44.08%。2008年下半年较2008年上半年3种病毒的检出率均有所升高,以AIV(H9)升高最为显著;2009年上半年较2008年上半年AIV(H9)阳性率上升最为明显。3种病毒混合感染的阳性率可达11.00%,以IBV+AIV(H9)和NDV+AIV(H9)二重混合感染临床最为常见。表明山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染。本调查结果为山东省制定规模鸡场3种疫病的防控措施提供了科学资料。Investigation on infections of NDV, IBV and AIV(H9) was performed by established RT-PCR technique. 1 837 suspicious samples collected from large-scale chicken farms in Shandong province (Weifang, Qingdao and Yantai) were detected during 2008.01 to 2009.06. The results showed that the positive rates of these three viruses was up to 65.76% totally in three districts and the positive rates of NDV, IBV and AIV(H9) were 12.36%, 12.30% and 41.10%, respectively. The positive rates of NDV(13.45%), IBV(17.37 %)and AIV(H9)(44.08%) were all highest in Qingdao. All of the positive rates of these three viruses were higher during 2008.07 to 2008.12 than during 2008.01 to 2008.06, especially for AIV(Hg). Chickens co-infected with dual and triple viruses accounted for 11.00% from the detected samples. The dual infection of IBV+AIV(H9) and NDV+AIV(H9) were most serious in all co-infectioned samples. The results demonstrated that NDV, IBV and AIV(Hg) were very prevalent in the commercial chickens in Jiaodong area. The investigation can offer the scientific evidence to prevent and control these diseases in Shandong province.
关 键 词:RT—PCR NDV IBV AIV 感染 调查
分 类 号:S858.31[农业科学—临床兽医学] S851.33[农业科学—兽医学]
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