Effect of 532 nm Laser Irradiation on Vc Content in Cabbage Seedlings  

532 nm激光辐照对白菜幼苗维生素C含量的影响(摘要)(英文)

作　　者：金丽虹[1] 申炳俊[1] 刘俊华[1] 赵新乐[1] 李景梅[1] 田坚[1] 

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130022

出　　处：《Agricultural Science & Technology》2009年第6期69-71,共3页农业科学与技术（英文版）

基　　金：Supported by Fund from Jilin Provincial Science & Technology Department(20090541);Project from Department of Education ofJilin Province(200828)~~

摘　　要：[ Objective ]The aim of this study was to discuss the feasibility of breeding cabbage with high Vc by 532 nm laser.[ Method ] With the material of cabbage, the embryo of cabbage seeds was irradiated by frequency doubled Nd ：YAG laser with different power densities and time, and the effects of laser mode on functional leaf area, chlorophyll and Vc contents in cabbage seedlings were also studied. [Result] The results showed that functional leaf area and chlorophyll content were related to laser power density and time when laser power density was 2 -20 mW/mm^2, while the optimal effect was observed at 14 mW/mm^2 for 1 min. The content of Vc in cabbage seedlings was related to dosage of laser irradiation when irradiating time ranged from 1 to 5 min, and the optimal effect was observed at 2.8 J （20 mW/mm^2, 3 min）. [Conclusion] Irradiating the embryo of cabbage seeds with proper irradiation dosage of 532 nm laser can increase its effect on the Vc content significantly.[目的]探讨532 nm激光选育高维生素C白菜的可行性。[方法]以白菜为实验材料,采用不同功率密度(2、6、10、14和20 mW/mm^2)和不同辐照时间(1、2、3、4和5 min)的Nd:YAG倍频激光对白菜种子胚进行辐照,研究激光作用方式对白菜幼苗功能叶片面积、叶绿素和维生素C含量的影响。功能叶片面积的测定:培养10 d时,取每颗白菜上最大的叶片,利用复印称重法对其面积进行测定,即将白菜叶片用激光复印机复印,沿叶片边缘剪下,准确称量其质量;用直径1.46cm的打孔器打50个纸圆片,算出单位面积纸张的质量(g/cm^2),计算后得出叶面积。叶绿素含量的测定:每组取白菜叶片10 g,以2 ml磷酸缓冲溶液(50 mmol/L,pH≈6.8)将其研磨成均质。吸取上清液40μl,注入1.5 ml的Eppendorf管中,再加入960μl的无水乙醇,置于4℃黑暗条件下30 min,然后于4℃下以1 000 r/min离心15 min。以质量分数为95%的乙醇做空白对照,利用U-2800分光光度计(日立)分别测定待测液的A_(665)和A_(649),由叶绿素总量=6.10×A_(665)+20.04×A_(649)来计算其含量。Vc含量的测定:利用库仑滴定法测定Vc含量,即在预电解后的电解池中准确加入2.0 ml样品溶液,开动搅拌器,按下库仑仪工作键进行10 mA恒电流电解,滴定终点时指示灯亮,记录电解所消耗电量,通过法拉第定律计算Vc含量。[结果]激光辐照功率为2～20 mW/mm^2时,功能叶片面积和叶绿素含量与激光辐照功率、作用时间有关。适当辐照功率、短时间激光处理白菜种子对功能叶片面积表现为刺激效应,当较高功率密度激光辐照时间超过一定阈值(为4 min)时,激光处理表现出抑制效应。短时间激光处理白菜种子对叶绿素含量表现为刺激效应,当激光辐照时间超过一定阈值(5 min)时,激光处理表现出抑制效应,其中以14 mW/mm^2激光预处理白菜种子1 min的效果最佳。激光辐照功率密度为2～10 mW/mm^2时,白菜Vc含量随辐照

关 键 词：532 nm laser irradiation Optimal irradiation dosage VC CHLOROPHYLL Functional leaf area 

分 类 号：TN249[电子电信—物理电子学]