Responses of Membrane Lipid Peroxidation and Endogenous Hormones of Soybean Seedlings to UV-B Radiation and Rare Earth  

植物膜脂过氧化作用和内源激素对UV-B辐射和稀土的响应(摘要)(英文)

作　　者：闫生荣[1,2] 杨春和[1] 张跃群[1] 花海蓉[1] 蒋云霞[1] 

机构地区：[1]南通农业职业技术学院环境与资源系,江苏南通226007 [2]江南大学环境与土木工程学院,江苏无锡214122

出　　处：《Agricultural Science & Technology》2009年第6期72-75,90,共5页农业科学与技术（英文版）

基　　金：Supported by the Foundation of State Developing and ReformingCommittee(No.IFZ20051210);the National Natural Science Foundationof China(No.30570323,No.20471030);the Programsin Science and Technology of Nantong(No.DE2009006,No.S2009019)~~

摘　　要：[ Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [ Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth were studied through hydroponics in laboratory. [ Result] The results showed that under irradiation of UV-B（ T1-0.15 W/m^2 and T2-0.45 W/m^2）, chlorophyll and indole-3-acetic acid（IAA） contents firstly decreased during the stress phase （1 -5 d） and then increased during the restoration phase （6 -9 d） while contents of malonadialdehyde（MDA） and abscisic acid（ABA） gradually increased during the imposition of UV-B radiation （1 -5 d） and subsequently decreased during recovery from UV-B stress （6 -9 d） . With adding of La （III） with the concentration of 20 mg · L^-1 , the decline/dse trend of chlorophyll, IAA, MDA and ABA contents was slowed down during the stress period while the rise/decline speed was accelerated during the recovery period. [ Conclusion] It suggests that the regulation of La （ III ） on membrane lipid peroxidation and endogenous hormones could increase chlorophyll and IAA contents, improve the metabolism of reactive oxygen species （ ROS）, inhibit membrane lipid peroxidation, decrease the accumulation amount of ABA and alleviate injury of UV-B radiation to soybean seedlings. Further, the protective potential of La （ III ） was better under low UV-B radiation than under high one.[目的]为稀土农用拓展及环境污染防治提供策略。[方法]采用水培法培养大豆,待第3枚三出复叶展开后进行La和UV-B辐射处理。以前期试验确定的最适浓度LaCl_3(20mg/L)溶液均匀喷洒大豆叶片,48 h后,放置于特定剂量的紫外灯下进行胁迫处理。UV-B设2个处理:T_1(0.15 W/m^2)、T_2(0.45 W/m^2),每天处理6 h(10:00-16:00),连续处理5 d,每天同一时间取同向、同叶位大豆幼苗叶片测定叶绿素(Ch1)、丙二醛(MDA)、H_2O_2膜脂脂肪酸、吲哚乙酸(IAA)和脱落酸(ABA)的含量。[结果]UV-B(T_1:0.15 W/m^2和T_2:0.45 W/m^2)辐射下,大豆幼苗叶绿素(Ch1)和吲哚乙酸(IAA)含量在胁迫期(1～5 d)逐渐下降,在恢复期(6-11 d)上升;丙二醛(MDA)和脱落酸(ABA)含量先增(1～5 d)后减(6～11 d)。20 mg/L La(Ⅲ)减缓了上述指标在胁迫期的下降/上升趋势,加快了恢复期的上升/下降速度。[结论]La(Ⅲ)对大豆幼苗膜脂过氧化和内源激素的调控能提高叶绿素和IAA含量,改善活性氧的新陈代谢,抑制膜脂过氧化作用,减少ABA积累,且对低剂量紫外辐射(T_1)的防护效果优于高剂量紫外辐射(T_2)。

关 键 词：La （ III ） UV-B radiation Soybean seedlings Membrane lipid peroxidation Endogenous hormones 

分 类 号：S184[农业科学—农业基础科学]