Dynamic of Soil Microorganisms from Root Region of Ginseng with Different Growing Years  

不同栽培年限的人参根区土壤微生物区系变化(摘要)(英文)

作　　者：李勇[1] 刘时轮[1,2] 易茜茜[1] 傅俊范[2] 丁万隆[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]沈阳农业大学植物保护学院,辽宁沈阳100161

出　　处：《Agricultural Science & Technology》2009年第6期141-143,共3页农业科学与技术（英文版）

基　　金：Supported by National Key Technology R & D Program(2006BAI09B04-01);Doctoral Fund of Ministry of Education ofChina(200800231060)~~

摘　　要：Objective To see the dynamic of fungi, bacilli and actinomyces communities from root region of ginseng with different growing years.Method With ginseng root region soils from several sampling sites of Jilin Province as materials, concentrations of fungi, bacilli and antinomyces were evaluated by spread-plate method. Result Though there are differences on statistic data among soil samples, commonly with the increasing of growing years, concentration of fungi in ginseng root region increased, which were on the contrary for bacilli and antinomyces, and bacilli changed even more significant than antinomyces. Conclusion Concentrations of soil microorganisms can be influenced by soil type, planting mode and growing years simultaneously, but growing years influenced even more significantly.[目的]明确不同栽培年限人参根区土壤真菌、细菌和放线菌浓度变化情况。[方法]以吉林省多个产地人参根区土壤作为研究对象,采用稀释平板法对真菌、细菌和放线菌进行分离和数量测定,真菌的分离用马丁培养基+孟加拉红+硫酸链霉素;细菌的分离用牛肉膏蛋白胨培养基;放线菌的分离用高氏1号培养基+重铬酸钾。真菌培养温度为25℃,培养时间为6～7 d;放线菌培养温度为28℃,培养时间为4～5 d;细菌培养温度为30℃,培养时间为2～4 d。根据不同培养基中生长出的菌落数统计真菌、细菌和放线菌数量,结合土壤样品的稀释倍数,按照公式:土壤微生物浓度(cfu/g)=(菌落平均数×稀释倍数)/每皿菌液加入量(ml),求得土壤中可培养真菌、细菌和放线菌浓度。[结果]不同地点采集的土壤样品的统计结果存在差异,但基本表现为随人参生长年限的增加,人参根区土壤中真菌浓度逐年升高,细菌和放线菌浓度逐年降低,且放线菌的变化不如细菌变化显著。[结论]土壤类型、种植模式及人参生长年限对农田土和林地土中3类微生物的浓度均有影响,但生长年限对人参根区土壤微生物的影响最显著。

关 键 词：GINSENG Root region soil FUNGI BACILLI ACTINOMYCES 

分 类 号：S567.51[农业科学—中草药栽培]