淡水鱼类GSTR基因微囊藻毒素活体诱导表达研究  

Transcriptional Responses of Rho-GST Gene in the Liver of Freshwater Fishes Intra-peritoneally Injected with Microcystin-LR

作　　者：黄燕[1] 

机构地区：[1]长治医学院心血管研究所,046000

出　　处：《长治医学院学报》2009年第6期401-403,共3页Journal of Changzhi Medical College

基　　金：广东省科技计划项目(2007B020701002;2005B20301005);广东省自然科学基金项目(031886);广州市科技计划(2006Z3-E0551)

摘　　要：目的:比较不同生态习性淡水鱼类对MC-LR的去毒能力与其肝脏Ⅱ相去毒酶GSTR基因诱导型表达之间的关系。方法:对实验鱼活体腹腔注射亚致死量MC-LR(50μg/kg),采用半定量RT-PCR方法,以β-肌动蛋白为外参照,测定不同生态习性淡水鱼类(如鲢鱼、尼罗罗非鱼、草鱼)肝脏8h、24hGSTR基因mRNA诱导表达水平。结果:对毒素耐受能力强的淡水鱼类(如鲢鱼、尼罗罗非鱼)8h肝脏GSTR表达升高,24h表达显著降低;而对微囊藻毒素高度敏感的淡水鱼类(如草鱼)8h、24h肝脏GSTR表达水平均无明显变化。结论:不同生态习性淡水鱼类肝脏GSTR基因诱导型表达的高低可能与其肝脏去毒能力有关,这将为深入探讨淡水鱼类MC-LR去毒相关基因的表达调控机制提供实验基础。Objective ： To compare the inducible expression level of the liver phase Ⅱdetoxified enzyme Rho - class GST gene to microcystin - LR in different freshwater fishes. Methods： Using beta - actin as external control, liver Rho - class GST gene in- ducible expression levels at 8 h and 24 h among silver carp （ Hypophthalmichthys molitrix ）, Nile tilapia （ Oreochromis nilotica ） and grass carp （Ctenopharyngodon ideUus ） were studied by RT - PCR, which were intraperitoneally injected with a single 50 μg/kg body weight （bwt） dose of pure MC - LR. Results： High - resistant fish （silver carp and Nile tilapia） which injected MC - LR showed significant induction（ P 〈 0.05） of GSTR over controls at 8 h,while significant reduced（ P 〈 0.05） at 24 h. However,in the high- sensitive fish （grass carp）,there is no significant difference of GSTR gene at 8 h and 24 h.Conclusion：The difference of inducible expression level in three freshwater fishes is relative to liver detoxification ability, which would provide experimental basis on expression and regulation mechanism of microcystin - LR detoxification - related genes in freshwater fishes.

关 键 词：微囊藻毒素 谷胱甘肽S-转移酶 肝脏表达 淡水鱼类 

分 类 号：Q786[生物学—分子生物学]