白介素1β对培养的大鼠皮层神经元钙通道电流的抑制作用  

Inhibition Effect of IL-1β on Calcium Channels Currents in Cultured Cortical Neurons of Rat

作　　者：周辰[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《Zoological Research》2010年第1期89-93,共5页动物学研究（英文）

基　　金：河北省教育厅科学研究计划(Z2009109);北京大学生物膜与膜生物工程国家重点实验室开放课题

摘　　要：白细胞介素1β(IL-1β)是重要的促炎细胞因子,在中枢神经系统(CNS)中发挥广泛的生物学功能。在病理条件下,细胞膜上电压门控钙通道的变化与疾病发展过程密切相关。虽然IL-1β和钙通道都在脑损伤和脑疾病过程中发挥重要作用,但目前还很少有两者之间相互关系的研究报道。该研究使用了培养的大鼠胎鼠皮层神经元和膜片钳记录技术,研究了长时间的IL-1β处理对电压门控钙通道电流的作用。结果表明,IL-1β在10和50ng/mL剂量下都可以抑制钙电流,这种抑制作用具有时间和剂量依赖性的模式,并且不改变钙通道的激活性质。Interleukin-1β（IL-1β） is an important porinflammatory cytokine that exert wide biological functions in the central nervous system （CNS）. Voltage-gated Ca^2＋ channels in plasma membrane are essential components of Ca^2＋ signal whose change on pathological conditions is closely related to processes of diseases. Although both IL-1βand Ca^2＋ channels have key roles in injury and disease of the brain, so far their relations have been reported in few studies. The effects of IL-1β on Ca^2＋ channels in cultured cortical neurons of rats were investigated using patch-clamp recording in present research. Our results showed that both 10 and 50 ng/mL of IL-1β treatment inhibit the Ca^2＋ currents in time and dose dependent manner, without the change of activation properties of voltage-gated Ca^2＋ channels （VGCC）s.

关 键 词：白介素1Β 钙电流 膜片钳记录 皮层神经元 

分 类 号：Q424[生物学—神经生物学] Q426[生物学—生理学]