标记辅助培育水稻抗稻褐飞虱和稻白叶枯病基因聚合系  

Rice Pyramiding Lines Conferring Resistances to Brown Planthopper and Bacterial Leaf Blight by Marker-Assisted Selection

作　　者：阳海宁[1,2] 韦绍丽[1,2] 李孝琼[1,2] 张月雄[1,3] 刘驰[1,3] 陈乔 岑贞陆[1,3] 韦素美[1,3] 杨朗[1,3] 黄凤宽[1,3] 何文强[1,2] 刘开雨[1,2] 黄大辉[1,3] 马增凤[1,3] 黄悦悦[1,2] 宋建东[1,2] 李容柏[1,2,3] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007 [2]广西大学广西亚热带生物资源保护利用重点实验室,生命科学与技术学院,农学院,南宁530005 [3]广西农科院水稻研究所,微生物研究所,植物保护研究所

出　　处：《分子植物育种》2010年第1期11-19,共9页Molecular Plant Breeding

基　　金：国家863计划课题(2006AA10Z1C5;2006AA100101-2;2007AA021405;2007AA10Z191);973前期基础研究专项(2007CB116305);国家自然科学基金课题(30960190);广西科学基金项目应用基础研究专项(桂科基0833025);广西科学研究与技术开发计划(桂科能05112001-1A1);广西科技创新能力建设项目(桂科能0630006-5F;桂科能0815011-6-1;桂科能090618);广西科学基金(桂科青0833078)共同资助

摘　　要：稻白叶枯病和稻褐飞虱是水稻的主要病虫害,利用优良的抗性基因培育抗性基因聚合系对于防治稻白叶枯病和稻褐飞虱具有重要意义。本研究通过回交与分子标记辅助选择相结合的方法,将抗稻白叶枯病基因Xa23和抗稻褐飞虱基因Bph3分别导入主栽杂交水稻品种的保持系先B、天B、盟B、龙特甫B和桂B中。结果表明,获得稳定的单抗性基因导入系1436份和双抗性基因聚合系144份。人工接种鉴定结果显示,Xa23导入系和Xa23Bph3聚合系对抗稻白叶枯水平达到中抗和接近高抗的水平(1.1～3.0级),Bph3导入系和Xa23Bph3聚合系抗褐飞虱水平达中抗到抗的级别(3.2～4.0级)。农艺性状分析显示多数抗性基因聚合系的株高、剑叶长宽度、每穗粒数、结实率、千粒重等主要农艺性状与受体差异不显著,只有少数聚合系有1～2个性状的差异。SSR标记分析表明抗性基因聚合系的遗传背景回复率达89.6%～97.8%,等位性位点纯合度达95.6%～99.9%。初步证明本研究已成功地获得抗稻白叶枯病和稻褐飞虱基因的聚合系,这些抗性改良保持系具有良好的应用前景,并为建立完善的分子设计育种平台提供了重要育种材料基础。Plant brownhopper （BPH, caused by Nilaparvata lugens （Stal）） and bacterial leaf blight （BLB, caused by Xanthomonas campestris PV. Oryzae） are two of the major diseases in rice. To breed pyramided lines of the two disease-resistance genes are of great importance for the control of the two diseases. In this research, BLB and BPH resistance genes, Xa23 and Bph3, were accumulated into 5 maintainers of current main cultivated rice hybrids of Guangxi, Xian B, Tianfeng B, Meng B, Longtepu B and Gui B, via backerossing together with molecular markerassisted selection （MAS）. As a result, we obtained 1 436 single resistance gene-inserted lines and 144 double resistance gene-accumulated lines. Data from the inoculation with the BLB pathogen and BPH insect showed that the Xa23-inserted lines and Xa23Bph3 accumulated lines were moderately resistant or near highly resistant to the BLB with resistance scores 1.1-3.0, while the Bph3-inserted lines and Xa23Bph3 accumulated lines were moderately resistant to the BPH with resistance scores 3.2-4.0. Agronomic traits analysis reveal that there were no significant differences between most of the gene-accumulated lines and receptor parents in plant height, flag leaf length and breadth, grain number per panicle, filled grained rate and 1 000-grain weight, except one or two traits in a few accumulated lines. SSR analysis showed that the recovery rate of the resistance gene-accumulated lines was up to 89.6%-97.8% of the receptors, and their allelic homozygosity reached 95.6%-99.9%. The above results preliminary demonstrated the BPH and BLB resistance accumulated lines were successfully bred and these resistance rice maintainers were quite potential in developing new promising resistant hybrid varieties. This work would also provide a solid foundation in breeding material for the establishment of the molecular design breeding platform.

关 键 词：杂交水稻 保持系 Bph3Xa23基因聚合 基因导入系 设计育种 

分 类 号：S511[农业科学—作物学]