活化小胶质细胞移植后损伤脊髓内TGF-β1mRNA、蛋白的表达及意义  

mRNA and protein expressions of TGF-β1 in spinal cord injuries and their significance after transplantation of activated microglia

作　　者：寇德伟[1] 于腾波[1] 李田米[2] 于振萍[1] 

机构地区：[1]青岛大学医学院附属医院关节外科,266003 [2]青岛大学医学院附属医院手术室,266003

出　　处：《中华创伤杂志》2010年第1期70-75,共6页Chinese Journal of Trauma

基　　金：山东省科技攻关计划资助项目（2006GG2302040）

摘　　要：目的探讨活化小胶质细胞移植后脊髓组织内TGF-β1mRNA和蛋白表达及意义。方法应用改良Allen法制造Wistar大鼠脊髓损伤模型，将活化小胶质细胞移植到脊髓损伤模型，测定脊髓组织内TGF-β1mRNA和蛋白表达。结果各时相点对照组TGF-β1mRNA及其蛋白表达量明显低于移植组（P〈0．01）。结论活化小胶质细胞移植后，可以上调损伤脊髓表达TGF—β1；脊髓内TGF—β1蛋白的高表达可能对脊髓损伤修复起促进作用；活化的小胶质细胞可能是TGF-β1的主要来源。Objective To investigate mRNA and protein expressions of TGF-β1 and their significance in injured spinal cord after transplantation of activated microglia. Methods Wistar rat model of spinal cord injury was made by using modified Allen＇ s method. Then, the activated microglia was transplanted into spinal cord injury models to detect mRNA and protein expressions of TGF-β1 in the spinal cord. Results The mRNA and protein expressions of TGF-β1 in control group were significantly lower than that in test group （ P 〈 0.05）. Conclusion Transplantation of activated microglia to the impaired spinal cord in rats can increase the mRNA and protein expressions of TGF-β1. The up-regnlated expression of TGF-β1 in spinal cord may play a role in promoting the recovery of spinal cord, when the activated microglia may be the main source of TGF-β1.

关 键 词：脊髓损伤 转化生长因子Β1 细胞移植 小神经胶质细胞 

分 类 号：R651.2[医药卫生—外科学]