我国部分地区牛支原体肺炎和关节炎的病原体诊断  

Etiological diagnosis of Mycoplasma bovis pneumonia and arthritis in some provinces in China

作　　者：冉智光[1] 谢建华[1] 骆璐[1] 杨泽林[1] 凌洪权[1] 苏承忠 熊仲良[1] 范伟兴[2] 米自由 

机构地区：[1]重庆市动物疫病预防控制中心,重庆渝北401120 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国预防兽医学报》2010年第1期40-43,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：重庆市科技攻关项目(2009AC1111)

摘　　要：重庆等4省市从外省引进的肉牛群发生以严重肺炎和关节炎为主要表现的疾病。该病的病变主要集中于肺脏,其组织学变化主要是间质增生,纤维素渗出,干酪样坏死,淋巴细胞、嗜中性粒细胞和单核细胞浸润。PCR检测病牛肺脏显示牛支原体阳性,而丝状支原体丝状亚种、牛分支杆菌和多杀性巴氏杆菌为阴性。从肺等组织中分离到牛支原体以及大肠杆菌、沙门氏菌、链球菌、葡萄球菌和烟曲霉,没有分离到巴氏杆菌。结果显示本病是以牛支原体感染为主引起的牛支原体肺炎和关节炎,长途运输等应激因素是该病突发的重要诱因,其他细菌和/或真菌继发感染加重了病情。A severe pneumonia and arthritis emerged in calve herds introduced from interstate to Chongqing and other three provinces. The disease caused lesions primarily in the lungs, with histopathological changes manifesting interstitial hyperplasia, fibrin exudation, caseous necrosis, and lymphocytes, neutrophils and monocytes infiltration. PCR detection showed that the affected lungs were positive for Mycoplasma bovis but negative for Mycoplasma mycoides subsp, mycoides, Mycobacterium bovis and Pasteurella multocida. In addition, Mycoplasma boris, E. coli, Salmonella, Streptococcus, Staphylococcus and Aspergillus fumigatus was successfully isolated from the lungs and other tissues, however, no Pasteurella was isolated. The results indicated that the disease was primarily caused by Mycoplasma bovis, which could have been triggered by long-distance transport and other stress factors; and the secondary infection of bacteria and/or fungus deteriorated the condition of the disease.

关 键 词：牛支原体 肺炎 关节炎 PCR 细菌分离 

分 类 号：S852.61[农业科学—基础兽医学]