芝芪菌质多糖的制备及其增强免疫活性的初步研究  

Preliminary study on immunoactivities of Zhiqi Fungal polysaccharides

作　　者：唐宁[1] 张敦林[1] 张李阳[1] 

机构地区：[1]南京晓庄学院生命科学系,江苏南京211171

出　　处：《中国预防兽医学报》2010年第1期68-70,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省教育厅自然科学基金资助项目(JHZD04-045)

摘　　要：为研究芝芪菌质多糖体外免疫活性增强作用,本研究采用水提、醇沉、离子交换层析等方法纯化芝芪菌质多糖,并对各多糖组分(GAP-1、GAP-2、GAP-3、GAP-3)进行小鼠脾细胞增殖、NK细胞活性以及小鼠腹腔巨噬细胞吞噬鸡红细胞活性的测定。结果表明:纯化的4种芝芪菌质多糖在10-3mg/mL～10-1mg/mL浓度范围内均能够显著增强ConA诱导的小鼠脾淋巴细胞增殖反应;在25mg/kg～100mg/kg剂量范围内均能够显著增强小鼠腹腔巨噬细胞吞噬鸡红细胞活性,而且呈剂量依赖性,其中以GAP-3效果最为明显;对NK细胞活性也有不同程度的增强作用,其中,GAP-3在浓度为100μg/mL时,NK细胞的杀伤率为27%,与IL-2阳性对照组比较,有显著增强作用(p<0.05)。本研究证明,纯化的芝芪菌质多糖具有较好的体外增强免疫活性,为芝芪菌质作为增强机体免疫力的饲料添加剂提供了理论依据。The enhancing immunoactivity of the Zhiqi （Ganoderma lucidum ＆ Astragalus membranaceus） fungal polysaccha.rides was investigated in mice. Four portions polysaccharides were tested and shown significant enhancement to ConA-induced spleen lymphocyte proliferation response and mouse peritoneal macrophage phagocytic activity to chicken erythrocyte. The NK cell activities were also enhanced, where the GAP-3 was shown to promote NK cell-killing rate up to 27 % at 100 μg/mL dose. These results provide an experimental basis for Zhiqi fungal substance as a feed additive to improve the animal immunity.

关 键 词：芝芪菌质 纯化多糖 免疫活性 

分 类 号：R392[医药卫生—免疫学]