回转器旋转对鸡胚脑细胞内游离Ca^(2+)水平的影响及其恢复  

Effects of Clinorotation on Cytosolic Free Calcium Level in Embryonic Chick Brain Cells.

作　　者：沈宏略[1] 陈雅[1] 孙彤[1] 张锦珠[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《航天医学与医学工程》1998年第6期447-450,共4页Space Medicine & Medical Engineering

摘　　要：探讨了不同胚龄鸡胚旋转不同时间后，脑细胞内游离Ｃａ２＋水平的变化。利用回转器模拟微重力生物效应及ｆｕｒａ－２比例荧光法，测定了孵化第６至１８ｄ鸡胚脑细胞游离Ｃａ２＋的浓度。结果显示：孵化第８至１７ｄ鸡胚经旋转不同时间后，其脑细胞内游离Ｃａ２＋浓度均有所下降，其中第１０ｄ鸡胚旋转４或７ｈ及第１３ｄ鸡胚旋转２４ｈ后，脑细胞内Ｃａ２＋浓度比对照组显著降低（Ｐ＜０．０１）。第１０ｄ和１３ｄ鸡胚分别旋转４ｈ和２４ｈ后再静置恢复相同时间，细胞内Ｃａ２＋浓度均有所增加，但仍低于对照组水平。结果表明：回转器旋转引起的鸡胚脑细胞内游离Ｃａ２＋水平降低是可逆的，而且在鸡胚脑后部的红色光灸。Cytosolic free Ca 2+ ([Ca 2+ ] i) in suspended brain cells of chicken embryos hatched for 6～18 d (E6-E18) was measured by using the fura 2/AM fluorescence spectroscoy.The biological effects of microgravity on the embryonic chicks were simulated by revolving on a clinostat during their hatch.The experimental results showed that level of [Ca 2+ ] i in E8-E17 embryonic brain cells decreased after revolving for different period.[Ca 2+ ] i in brain cells of E10 and E13 chick embryos reduced significantly (P<0.01) after revolving 4～7 h and 24 h respectively. When E10 and E13 chick embryos were incubated again as long as being revolved,[Ca 2+ ] i in brain cells rose but were still lower than the controls. The levels of cytosolic free calcium in brain cells of revolved E17 embryonic chicks increased after being colorpunctured by red light. These results demonstrate that the decrease of [Ca 2+ ] i in embryonic chick brain cells caused by clinorotation is reversible,and colorpuncture can accelerate this recovery.

关 键 词：回转器 微重力 生物效应 钙离子 脑细胞 胚细胞 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]