发酵过程中菌液浓度的检测  

Measurement of Cell Concentration in Fermentation Liquid

作　　者：杨帆[1] 李淑梅[2] 陈萍[1] 李进芬[1] 

机构地区：[1]新乡医学院微生物学教研室,河南省新乡市华兰大道东段453003 [2]河南科技学院化学化工学院,河南省新乡市华兰大道东段453003

出　　处：《光谱实验室》2009年第6期1643-1645,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：采用分光光度测量发酵菌液浓度,选择波长215nm,首先测定样品中菌液的吸光度,再根据校准曲线获得菌体浓度。该法设备简单,操作方便、快速,测量相对误差小于3%,相对标准偏差小于2%,精密度高,准确度好,适合于生产监控。The cell concentration in fermentation liquid was determined by spectrophotometry at 215nm. According to the linear relationship between the cell concentration and its absorption in some range, the cell concentration was obtained. This method is simple, easy, quick, and accurate, and is adaptable to control practical production.

关 键 词：分光光度法 菌液浓度 

分 类 号：O657.32[理学—分析化学]