大鼠肛门水肿中药坐浴治疗的实验研究  

Experimental Study of Rehabilitation of Rats with Anal Edema with Traditional Chinese Medicine

作　　者：郭秀君[1] 秦娟[3] 赵小寅 卞玉花[2] 杜媛[2] 金翔[5] 

机构地区：[1]南京中医药大学第三附属医院护理部,210001 [2]南京中医药大学第三附属医院肛肠科,210001 [3]南京中医药大学护理学院 [4]南京中医药学会 [5]南京中医药大学药学院

出　　处：《中华现代护理杂志》2009年第36期3860-3864,共5页Chinese Journal of Modern Nursing

基　　金：南京市科学技术局社会发展项目（200702070）

摘　　要：目的通过中药坐浴不同干预时段和持续时间对大鼠肛门水肿恢复的研究，为临床探讨最佳疗效的中药坐浴方法提供依据。方法采用注射角叉菜胶致大鼠肛门水肿模型，将96只大鼠分早期干预及晚期干预2批，每批随机分为6组：空白1组、2组；模型1组、2组；给药1组、2组；运用中药坐浴的方法，1组坐浴持续时间为10min，2组坐浴持续时间为20min，观察肛门水肿的恢复情况。结果中药坐浴早期干预在大鼠肛门水肿面积消退、干湿重比值、微循环变化、皮肤温度指标中与模型组比较差异均有统计学意义（P〈0．05），且坐浴效果20min组优于10min组（P〈0．01）；中药坐浴晚期干预仅在局部微循环变化及干湿重比值指标中与模型组比较差异有统计学意义（P〈0．05）。病理组织学检查给药组在局部组织炎性细胞浸润、组织间隙疏松程度及血管充血方面均较模型组有所改善。结论中药坐浴早期于预对大鼠肛门水肿的恢复有明显的效果，能促进肛门水肿的消退，改善水肿部位微循环，降低水肿局部皮肤温度，缓解肿胀区域炎症充血症状，且多项指标显示，坐浴20min的效果优于10min。Objective To investigate the effects of different time duration hip bathing with traditional Chinese medicine for rehabilitation of rats with anal edema, which can provide evidence for a best effective hip bathing method in the clinical application. Methods The rat model of anal edema was induced with carrageenan injection. Ninty six SD rats were divided into early intervene and latter intervene batches, every batches were randomly divided into six groups： normal control groupl, normal control group2, model matched groupl, model matched group2, hip bathing with TCM groupl, hip bathing with TCM group2. Hip bathing time duration were 10minutes and 20minutes. The rehabilitation of rats anal edema were observed. Results The comparison has been made among edema area, the ratio of dry/wet weight, microcirculation change, skin temperature,the difference is statistically significant in TCM early intervene hip bathing groups （ P 〈 0. 05 ）. There was a significant difference of edema proportion with 20 minutes duration between TCM hip bathing groups and model control groups （ P 〈 0. 01 ）. The comparison has been made among the ratio of dry/wet weight, microcireulation change, the difference is statistically significant in TCM latter intervene hip bathing groups（P 〈 0. 05 ）. Histopathological examination showed local tissue inflammatory cell infiltration, the loosen degree of tissue space and capillary congestion of modle group were improved. Conclusions Hip bathing with traditional Chinese medicine in early intervene have obvious effect on rehabilitation of rats with anal edema, which can dispetl anal edema, improving microcirenlation could descend the temperature and promote the rehabilitation of anal edema, relieve inflammation and hyperemia. In the respect of edema proportion relieving, hip bathing with 20 minutes duration showed better effect than 10 minutes.

关 键 词：肛门水肿 坐浴 中药 动物实验 

分 类 号：R244.9[医药卫生—针灸推拿学]