结缔组织生长因子siRNA抑制高糖诱导的大鼠系膜细胞合成结缔组织生长因子及Ⅰ型胶原的表达  

ibitory effects of connective tissue growth factor siRNA in high glucose induced connective tissue growth ：or and collagen synthesis in rat mesangial cells

作　　者：于海[1] 周春华[1] 

机构地区：[1]海军总医院肾脏病科,北京100048

出　　处：《中国医药》2010年第2期125-127,共3页China Medicine

摘　　要：目的研究针对结缔组织生长因子（CTGF）的小于涉RNA（siRNA）对高糖诱导大鼠系膜细胞（RMC）合成CTGF及Ⅰ型胶原（collagentypeⅠ）的抑制作用。方法设计并合成针对CTGF的siRNA。以含有不同浓度葡萄糖（5、10、15、20、25mmol／L）的培养基培养RMC。将CTGFsiRNA转染至高糖（25mmol／L）环境刺激的RMC中，以RT—PCR和western blot检测siRNA对RMC合成CTGF及Ⅰ型胶原的影响。结果随着培养液中葡萄糖浓度的不断升高（最高浓度为25mmol／L），RMC合成CTGF及I型胶原明显增加，转染针对CTGF的siRNA后，CTGF及Ⅰ型胶原的合成较对照组明显减弱，与正常葡萄糖浓度组水平相当。结论针对CTGF的siRNA能明显抑制高糖诱导的RMC合成CTGF及Ⅰ型胶原。Objective To investigate the inhibitory effects of siRNA targeting CTGF on the expression of CTGF and collagen type Ⅰ in cultured rat mesangial cell （RMC） with high glucose. Methods SiRNA targeting CTGF was designed, synthesized and transfected into RMC cultured with high glucose. Expression of CTGF and collagen type I was examined by reverse transcription polymerase chain reaction（RT-PCR） and western blot. Results After siRNA was transfected, expression of CTGF and collagen type Ⅰ decreased significantly compared with that in RMC cultured with only high glucose. Conclusion The siRNA targeting CTGF could inhibit synthesis of CTGF and collagen type Ⅰ in RMC cultured with high glucose.

关 键 词：糖尿病性肾病 RNA干涉 结缔组织生长因子 大鼠系膜细胞 

分 类 号：R587.24[医药卫生—内分泌]