玉米小斑病菌T小种毒素诱导对玉米叶片苯丙氨酸解氨酶活性的影响  

Effect of Inducement by HMT-Toxin to PAL Activity in Maize Leaves

作　　者：马春红[1] 翟彩霞[2] 郑秋玲[3] 董文琦[4] 李运朝[1] 崔四平[1] 范尉尉[5] 贾银锁[1] 

机构地区：[1]河北省农林科学院遗传生理研究所,石家庄050051 [2]河北省农林科学院农业资源环境研究所,石家庄050051 [3]河北省农林科学院滨海农业研究所,唐海063200 [4]河北省农林科学院科技处,石家庄050051 [5]河北省石家庄市疾病预防控制中心,石家庄050011

出　　处：《华中农业大学学报》2010年第1期21-25,共5页Journal of Huazhong Agricultural University

基　　金：科技部国际科技合作项目(2006DFB02480);农业部948项目(2008-Z20);河北省自然科学基金项目(C2006000744);河北省科学技术研究与发展计划项目(07297162D)资助

摘　　要：分别采用低浓度玉米小斑病菌Hel minthospolium maydis T小种毒素(HMT毒素)和玉米小斑病菌T小种培养滤液中的提取蛋白及菌丝细胞壁的提取液,预处理不同玉米品种3叶期叶片后再接种高浓度HMT毒素,在无菌条件下培养5 d后测量叶片病斑大小,并测定玉米叶片内苯丙氨酸解氨酶(PAL)活性变化。结果表明:只有纯化的低浓度HMT毒素能够作为激发子诱导玉米获得系统性广谱抗性,但不同玉米品种所需的最适合浓度有差异;TC103以质量浓度0.025μg/mL毒素预处理效果最好,PAL活性最高;TB37和TMo17 2个自交系,以质量浓度分别为0.025μg/mL和0.050μg/mL的毒素预处理效果最好。The maize leaves were pretreated respectively by low concentraion Helrninthospoliurn rnaydis T-toxin（HMT-T）, protein and cell-wall, which were extracted from cultivating filtrate at the stage of three-leaf, and then these leaves were treated by high concentration HMT-T and cultivated 5 d. The dimension of spots in infected leaves was calculated and phenylalanine ammonia lyase（PAL） activity determined. The result showed that only the HMT-T could act as elicitor in the cultivating filtrate. The suitable concentration of HMT-T was different for various varieties. As to TC103 the suitable concentration of toxin was 0. 025 μg/mL and the PAL activity of maize leaves reached its maximum degree in this case. The right concentration of TB37 and TMol7 were 0. 025μg/mL and 0. 050μg/mL respectively.

关 键 词：玉米小斑病菌T小种 毒素 激发子 苯丙氨酸解氨酶 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]